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Registration Dossier
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EC number: 230-363-2 | CAS number: 7069-42-3
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study conducted in accordance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- Retinyl propionate
- EC Number:
- 230-363-2
- EC Name:
- Retinyl propionate
- Cas Number:
- 7069-42-3
- Molecular formula:
- C23H34O2
- IUPAC Name:
- retinyl propionate
- Details on test material:
- - Vitamin A-propionat, (retinyl-propionate)
- Purity: 95.9 w -%
- Batch No.: 00000777LO PBG-Nr.10540158
- Water solubility: < 10 ug/L at 20.0 °C ± 0.1 °C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: activated sludge obtained from a laboratory wastewater plant treating municipal sewage
- Initial biomass concentration: 1 g/L dry matter
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, total volume, test volume: Erlenmeyer-vessel, 250 ml, 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- Oxygen concentration during aeration: > 2.5 mg/l
- Oxygen concentration immediately before measurement: > 6.5 mg/l
- Duration of the measurement of oxygen consumption: 8 - 10 min - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (500 mg/L)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- Effect concentrations after 180 min (nominal):
EC20 = ca. 1.2 mg/L
EC50 = ca. 18 mg/L
EC80 = ca. 50 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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