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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts
EC Number:
272-238-5
EC Name:
Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts
Cas Number:
68784-31-6
Molecular formula:
C(16-24) H(36-42) O4 P2 S4 Zn
IUPAC Name:
Phosphorodithioic acid, mixed O,O-bis(sec-Bu and 1,3-dimethylbutyl) esters, zinc salts

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source:
- Age at study initiation: 2.346 to 2.608 kg
- Weight at study initiation:
- Housing: Individually in wire mesh suspension cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 7 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17.8-26.1
- Humidity (%): 40-70
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): 12 hours


IN-LIFE DATES: From: To:

Test system

Vehicle:
unchanged (no vehicle)
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 ml
- Concentration (if solution):


VEHICLE
- Amount(s) applied (volume or weight with unit):
- Concentration (if solution):
- Lot/batch no. (if required):
- Purity:
Number of animals or in vitro replicates:
6 (non rinsed) and 3 (rinsed)
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing (if done): rinsing with 200 ml of lukewarm tap water was done on 3 of 9 animals
- Time after start of exposure: 30 seconds


SCORING SYSTEM: Draize


TOOL USED TO ASSESS SCORE: Fluorescein sodium ophthalmic solution U.S.P. followed by UV illumination

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24-72 hours
Score:
1.67
Reversibility:
not reversible
Remarks on result:
other: Non-rinsed
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 24-72 hours
Score:
1.22
Reversibility:
fully reversible
Remarks on result:
other: Rinsed
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24-72 hours
Score:
1
Reversibility:
fully reversible within: 14 days
Remarks on result:
other: Non-rinsed
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24-72 hours
Score:
0.22
Reversibility:
fully reversible
Remarks on result:
other: Rinsed
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24-72 hours
Score:
2.22
Reversibility:
not reversible
Remarks on result:
other: Non-rinsed
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24-72 hours
Score:
2
Reversibility:
fully reversible
Remarks on result:
other: Rinsed
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24-72 hours
Score:
2.78
Reversibility:
not reversible
Remarks on result:
other: Non-rinsed
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24-72 hours
Score:
2.11
Reversibility:
fully reversible
Remarks on result:
other: Rinsed

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria
Remarks:
Migrated information