Dimethyl cyclohexane-1,4-dicarboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study but conducted using nominal concentrations and not performed under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: EPA 660/3-75-009, Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EPA 600/4-85/013, Methods for measuring the acute toxicity of effluents to freshwater and marine organisms, 3rd ed.

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethyl cyclohexane-1,4-dicarboxylate (trans)

Cas Number:

3399-22-2

Molecular formula:

C10H16O4

IUPAC Name:

1,4-dimethyl cyclohexane-1,4-dicarboxylate (trans)

Details on test material:

- Name of test material (as cited in study report): trans-DMCD
- Lot/batch No.: 910521

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Test specimens of Daphnia magna originated from in-house cultures maintained in YMA 's aquatic toxicology laboratory. To prepare for test initiation, gravid adults of Daphnia magna were isolated from the mass cultures the day before study initiation. Neonates discharged from the isolated adults were collected on the following day for use in the tests.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

Dilution water hardness = 82 mg/L

Test temperature:

24 ± 1C

pH:

7.7-8.1

Dissolved oxygen:

7.8-8.2

Nominal and measured concentrations:

0, 10, 18, 32, 56, & 100 mg/L nominal only

Details on test conditions:

Each definitive test was conducted using at least five concentrations of trans-DMCD plus a dilution water control. Two replicate test vessels per treatment level were used, with each replicate holding 10 test organisms (n = 20/treatment level). The tests were each conducted in 100-ml glass containers holding 50 ml of test solution. Each test was considered initiated with the completion of the distribution of test organisms to their respective test vessels. Impartial distribution was performed by sequentially adding one to two organisms to each test chamber until all test chambers had their complement of 10 organisms. Distribution was completed within 30 minutes of the preparation of exposure solutions. All test vessels were placed in an environmental chamber at 24+ 1C under 50-100 ft-candles of light and a 8-hour dark/16-hour light photoperiod. Mortality and any abnormal effects on daphnids were recorded at 0, 24 and 48 hours. The LC50 and 95% confidence intervals were determined, if possible, for each test.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No toxicity to Daphnia magna was seen at concentrations up to 100 mg/L. Only one individual daphnid died during the toxicity test at the 100 mg/L concentration. For this reason, neither an LC50 nor an LC10 could be determined, but was considered > 100 mg/L, the highest concentration used in
the test.

Applicant's summary and conclusion

Executive summary:

The aquatic toxicity of trans-DMCD was determined for Daphnia magna in a 48-hour static test conducted according to EPA 660/3-75-009, Methods for acute toxicity tests with fish, macroinvertebrates and amphibians and EPA 600/4-85/013, Methods for measuring the acute toxicity of effluents to freshwater and marine organisms, 3rd ed. The test was conducted using five nominal concentrations of trans-DMCD plus a dilution water control. Two replicate test vessels per treatment level were used, with each replicate holding 10 test organisms (n = 20/treatment level). No control mortality occurred during the 48-hour test. One death was seen in the 100 mg/L concentration. For this reason, neither an LC50 nor an LC10 could be determined, but was considered > 100 mg/L, the highest concentration used in the test.