Benzoic acid, 2-amino-5-fluoro-, methyl ester

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP- and Guideline-Study with no or minor deficiencies.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: ISO/DIS 10712 (1994)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

methyl 2-amino-5-fluorobenzoate

EC Number:

608-681-1

Cas Number:

319-24-4

Molecular formula:

C8 H8 F N O2

IUPAC Name:

methyl 2-amino-5-fluorobenzoate

Test material form:

other: light yellow solid

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Test conditions

Test temperature:

21 °C

pH:

7.5

Nominal and measured concentrations:

0 (Ctrl), 7.8, 15.6, 31.3, 62.5, 125, 250, 500, 1000 mg/l nominal

Reference substance (positive control):

yes

Remarks:

2,4 Dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

EC10

Effect conc.:

10 - 100 mg/L

Effect concentrations 2

Duration:

16 h

Dose descriptor:

EC50

Effect conc.:

100 - 1 000 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the test item is in the range of 100 mg/l- 1000mg/l.