Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 266-120-2 | CAS number: 66072-08-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Under the conditions of this study, exposure of Daphnia magna to the test material gave a 48 hour EL50 value based on nominal loading rates of 2.4 mg/L; 95 % confidence limits 1.8 - 3.2 mg/L loading rate WAF.
As a result of the EL50 value, in conjunction with the substance's physico-chemical properties, the test material requires classification for being hazardous to the aqueous environment and as such is categorised as Chronic Category 2 in accordance with EU criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.4 mg/L
Additional information
The acute toxicity potential of the test material to Daphnia magna was assessed in the key study in accordance with the standardised guidelines OECD 202 and EU Method C.2 under GLP conditions. It was assigned a reliability score of 1 in accordance with the criteria set forth in Klimisch (1997).
Due to the low aqueous solubility and complex nature of the test material, it was prepared as a Water Accommodated Fraction (WAF).
Following preliminary range-finding tests, twenty daphnids (4 replicates of 5 animals) were exposed to WAFs of the test material over a range of nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 48 hours at a temperature of approximately 21 °C under static test conditions. The number of immobilised Daphnia and any adverse reactions to exposure were recorded after 24 and 48 hours.
Total Organic Carbon (TOC) analysis of the test preparations at 0 hours showed concentrations ranging from less than the Limit of Quantitation (LOQ), assessed to be 1.0 mg C/L, to 5.51 mg C/L and at 48 hours showed concentrations ranging from less than the LOQ to 5.60 mg C/L.
Given that the toxicity cannot be attributed to a single component or a mixture of components, but to the test item as a whole the results were based on nominal loading rates only.
Under the conditions of this study, exposure of Daphnia magna to the test material gave a 48 hour EL50 value based on nominal loading rates of 2.4 mg/L; 95 % confidence limits 1.8 - 3.2 mg/L loading rate WAF.
As a result of the EL50 value, in conjunction with the substance's physico-chemical properties, the test material requires classification for being hazardous to the aqueous environment and as such is categorised as Chronic Category 2 in accordance with EU criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
