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EC number: 203-505-6 | CAS number: 107-58-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental result using OECD recommended guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was conducted to assess the effect of the test compound on fish Danio rerio.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Degree of purity/content : 99.41%
Physical state/appearance : Solid
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
Storage conditions : Room temperature 30°C
Stability : Substance is stable under experimental conditions
Solubility in water : Soluble
Stability in water : Stable - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Preparation of the test solutions/dispersions : The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring.
Test water : Deionized water was used.
Conductivity (µS/cm): 0.5 to 1
pH: 7 - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name:Zebra Fish
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): average 2.3 cm
- Weight at study initiation (mean and range, SD): 0.203 g (approx.)
- Housing :The fishes were kept in a static tank in tap water, under natural conditions along with proper feed and aeration.
- Duration of housing : 15 days
- Oxygen content : 7.4
- pH : Between 6-7
- Water temperature : 29 °C
- Mortality (%) :0%
- Diet : Standard brand feed, once daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 29°C
- pH:
- 6-7
- Dissolved oxygen:
- 7.4
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12” each.
- Aeration: Aeration was provided 1 hr before the starting of experiment and also during the experiment when required.
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Photoperiod:12 hours light and 12 hours darkness
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50 was determined.Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 1st, 2nd, 3rd and 4th day of the start of the experiment.
TEST CONCENTRATIONS : Test was conducted at 100 mg/L to observe the adverse effect/ no effect of test substance on fresh water fish. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: LC0 was observed at 100 mg/l
- Details on results:
- The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria.
- Sublethal observations / clinical signs:
OBSERVATIONS:
Table 1. Mortality and symptoms
Nominal conc. (mg/l)
Number of fish
Mortality (cumulated) after
1stDay
2ndDay
3rdDay
4thDay
0 (control)
8
0
0
0
0
100
8
0
0
0
0
Nominal conc. (mg/l)
Number of fish
Symptoms after
1stDay
2ndDay
3rdDay
4thDay
0 (control)
8
NS8
NS8
NS8
NS8
100
8
NS8
NS8
NS8
NS8
Explanation of symptoms:
A: apathy
N: narcotic-like state
C: swimming near water surface
D: swimming at the bottom of bowl
M: Slow movements compared to control
NS: No abnormal symptoms
Number behind symbol for symptom= number of affected fish
Table 2. pH and oxygen content:
Nominal conc. (mg/l)
Number of fish
pH Readings after
1stDay
2ndDay
3rdDay
4thDay
0 (control)
8
6.4
6.8
6.7
6.1
100
8
6.5
6.6
6.5
7.3
Nominal conc. (mg/l)
Number of fish
Oxygen content (mg/l) after
1stDay
2ndDay
3rdDay
4thDay
0 (control)
8
7.4
7.1
6.1
6.5
100
8
7.5
7.2
6.2
6.5
Table 3. Test temperature:
Nominal conc. (mg/l)
Number of fish
Temperature (°C) after
1stDay
2ndDay
3rdDay
4thDay
0 (control)
8
28
28
29
30
100
8
28
28
29
30
- Validity criteria fulfilled:
- yes
- Conclusions:
- The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l.
- Executive summary:
This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Reference
Description of key information
This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.
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