Registration Dossier

Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study is a preliminary internal testing conducted for the water solubility study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report Date:
2012

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The principle of the method is a preliminary testing to determine the stability of the substance in neutral water.
GLP compliance:
no
Remarks:
The study has been conducted to determine the duration of stability of the substance in neutral water for the water solubility study

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
Name : Phosphorous acid, triC12-C14-alkyl esters
Batch 81380x062
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material :
no further details
Radiolabelling:
not specified

Study design

Analytical monitoring:
no
Duration of test
Duration:
72 h
Temp.:
30 °C
Initial conc. measured:
ca. 10 other: mg/ml
Number of replicates:
3 flasks have been prepared for the repeatability of the study.
Each of the 3 solutions is analyzed by chromatography at 0h, 1h, 4h, 8h, 16h, 24h, 48h and 72h .
Positive controls:
not specified
Negative controls:
not specified

Results and discussion

Preliminary study:
A visual observation of the sample over time indicates that the substance decomposes quite rapidly in water, after 24h. Analytical measurements indicate that the substance is stable for a duration of 24h (100% recovery at 24h) but starts to degrade after 24h. Then, the clear and transparent solution becomes cloudy indicating the degradation of the substance. At 48h, 10.7 % of the substance is recovered and at 72h only 8.5 % remain in the solution.
Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 203-982-0]
IUPAC name:
dodecan-1-ol
Identity:
1-Dodecanol
Identity:
dodecan-1-ol
Identity:
lauryl alcohol
CAS number:
112-53-8
Molecular formula:
C12H26O
Molecular weight:
186.334
SMILES notation:
CCCCCCCCCCCCO
InChl:
InChI=1/C12H26O/c1-2-3-4-5-6-7-8-9-10-11-12-13/h13H,2-12H2,1H3
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
tetradecanol
No.:
#3
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 233-663-1]
IUPAC name:
phosphorous acid
CAS number:
10294-56-1
Details on hydrolysis and appearance of transformation product(s):
After 24 hours of dissolution in water, the clear and transparent solutions become cloudy. When the solution is injected in the chromatography analyzer, a degradation of the substance is observed.
This observation highlights the unstability of the substance in water between 24h and 48h.
Total recovery of test substance (in %)open allclose all
% Recovery:
ca. 100
pH:
5.6
Temp.:
30 °C
Duration:
ca. 24 h
% Recovery:
ca. 10.7
pH:
4.7
Temp.:
30 °C
Duration:
ca. 48 h
% Recovery:
ca. 8.5
pH:
4.8
Temp.:
30 °C
Duration:
ca. 72 h
Dissipation DT50 of parent compound
Type:
not specified
Remarks on result:
not determinable because of methodological limitations
Details on results:
The degradation of the substance is observed after 24h and drop from 100% at 24h to 10.5% at 48h.
According to the chemical reaction, the major degradation products are expected to be phosphorous acid, dodecan-1-ol and tetradecanol

Any other information on results incl. tables

3 flasks have been prepared to study the substance stability in water (concentration : 100 mg/10 ml of water) according to the methodology described above.The results are presented below :

 

Stirring Time

Sample
(g)

Recovery
(%)

Observation

0h

0,1068

100

2 distinct phases

pH = 8

0h

0,1101

1h

0,1010

103

The solution is clear with droplets at the surface

 

 

 

1h

0,1002

1h

0,0967

4h

0,1132

101

4h

0,1103

4h

0,1083

8h

0,1067

101

8h

0,1059

16h

0,1069

102

16h

0,1018

16h

0,0997

24h

0,1035

100

 

pH = 5.6

24h

0,1049

24h

0,1033

48h

0,1103

10.7

Clouded solution

pH = 4.7

48h

0,0967

48h

0,1020

72h

0,1112

8.5

Clouded solution

pH = 4.8

72h

0,1112

72h

0,1052

 

The substance is stable during 24h in water at a concentration of 100mg/100ml, under continous stirring.

After 24 hours, the substance degrades significantly and the solution becomes clouded.

The chemical reaction occuring is the degradation of phosphorous acid, tri-C12-C14 alkyl esters and probably the formation of the following degradation products: phosphorous acid, dodecan-1-ol and tetradecanol.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The stability study indicates that the substance remains stable in water during 24 hours a concentration of 100mg/100ml, but degrades significantly between 24h and 48h.
According to the chemical reaction, it is expected that the degradation leads to the formation of the following degradation products : phosphorous acid, dodecan-1-ol and tetradecanol.