1,1,1-trichloroethane

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study conducted by reputable laboratory

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC 84/449/EEC, Annex 5, B1

Deviations:

no

GLP compliance:

yes

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1-trichloroethane

EC Number:

200-756-3

EC Name:

1,1,1-trichloroethane

Cas Number:

71-55-6

Molecular formula:

C2H3Cl3

IUPAC Name:

1,1,1-trichloroethane

Details on test material:

-Name of test material (as cited in study report): BALTANW D (solvant du Foraperle 219). Stabilised 1,1,1-trichloroethane.
- Storage condition of test material: 4 0C
-No other details given:

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source:Charles River, France
- Weight at study initiation: males 162 g and females 146 g
- Housing: 5 rats of the same sex per polycarbonate cage
– Diet: ad libitum A04C pelleted diet (U.A.R., 91360 Villemoisson sur Orge, France)
- Water : ad libitum drinking water filtered by a Millipore membrane (0.2µm)
- Acclimation period: > 5days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-3°C
- Humidity (%): 30-70%. 12
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: October 8th 1985

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

unchanged (no vehicle)

Doses:

2.0 ml/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs recorded once daily (with increase frequency in the first 3 hours after administration), animals weighed on days 1, 5, 8 and 15
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

> 2 000 mL/kg bw

Remarks on result:

other: no mortalities

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

< 2 000 mL/kg bw

Remarks on result:

other: 4/5 mortalities

Mortality:

Four male mortalities at the highest dose tested (2 ml/kg). On Days 1, 2 and 10

Clinical signs:

After dose administration all animals appeared sedated, had loss of balance and pilo-erection. 3 hours after administration females showed pilo-erection only and had recovered by Day 2.Surviving males had no clinical signs by Day 4.

Body weight:

All females showed continued weight gain during the 14 day observation period. One surviving male showed reduced weight gain on Days 5 and 8: this animal died on Day 10. The surviving male showed acceptable weight gain throughout the observation period

Gross pathology:

The male that died on Day 10 had haemorrhaging of the intestine. No other macroscopic findings were recorded at necropsy.

Applicant's summary and conclusion

Conclusions:

Under these experimental conditions, the oral LD50 of 1,1,1-trichloroethane is > 2 ml/kg for females and less than 2 ml/kg in males, following intra-peritoneal administration.

Executive summary:

The Acute intra-peritoneal toxicity of 1,1,1-trichloroethane was evaluated in male and female rats in compliance with principles of Good Laboratory Practices. Animals were treated and then observed for 14 days for mortality, clinical signs and effect on body weight.

No female mortality was recorded in the 14 day-observation period, but 4/5 males had died by Day 10. Transient clinical signs (sedation, loss of balance, pilo-erection) were o served in both sexes.  Females showed no effect on body weight.

Under these experimental conditions, the oral LD50 of 1,1,1-trichloroethane is > 2 ml/kg for females and less than 2 ml/kg in males, following intra-peritoneal administration.