Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 214-787-5 | CAS number: 1194-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 August 1984 to 02 May 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1984
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.2 (Acute Toxicity (Inhalation))
- Deviations:
- yes
- Remarks:
- (only two test concentrations)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-3 (Acute inhalation toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Limit test:
- yes
Test material
- Reference substance name:
- Dichlobenil
- EC Number:
- 214-787-5
- EC Name:
- Dichlobenil
- Cas Number:
- 1194-65-6
- Molecular formula:
- C7H3Cl2N
- IUPAC Name:
- 2,6-dichlorobenzonitrile
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Physical state: solid
- Storage condition of test material: room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Housing: stainless steel wire mesh cages
- Diet: ad libitum
- Water: tap water ad libitum
- Acclimation period: 6-8 days
ENVIRONMENTAL CONDITIONS
- Temperature: 20-25 °C
- Humidity: 48-77 %
- Air changes: 26 times per hour
- Photoperiod: 12 hours light / 12 hours dark
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose only
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure chamber volume: 40 L
- System of generating particulates/aerosols: Rehma type 504104 nebulizer
- Method of particle size determination: measured using a Marple personal cascade impactor, model 296. Samples were taken through the inlet adaptor at nose level at 1.5 L/min at 105 and 165 minutes after the start of exposure. Directly after sampling, the filters were extracted with methanol and the test material measured spectrophotometrically.
- Temperature, humidity, pressure in air chamber: 23.4-23.8 °C; 60.1-65.0 %
TEST ATMOSPHERE
- Brief description of analytical method used: samples of test atmosphere were taken through a 6cm plastic tube at 2 L/min for 2 minutes. After sampling, filters and the silica were extracted with dichloromethane and amounts of test material measured spectrophotometrically.
- Samples taken from breathing zone: yes
TEST ATMOSPHERE (if not tabulated)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): 2.11-2.57 µm (105 minutes); 2.00-2.61 µm (165 minutes) / 2.89-3.13 (105 minutes); 2.90-3.14 (165 minutes) - Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 240 min
- Concentrations:
- 0, 540 and 700 mg/m^3 (nominal)
133-233 mg/m^3 (lowest dose) - 250-326 mg/m^3 (highest dose) - No. of animals per sex per dose:
- Five
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: during exposure, respiratory frequencies if two males and two females were recorded; At 1.5, 18, 24 and 48 hours after exposure each animal was checked for toxic symptoms and then observed daily
- Necropsy of survivors performed: yes
- Other examinations performed: organ weights (lungs and trachea) and organ histopathology
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 250 mg/m³ air (analytical)
- Based on:
- test mat.
- Remarks on result:
- other: maximum attainable concentration (limit dose)
- Mortality:
- No deaths were reported during the study.
- Clinical signs:
- other: A temporarily decreased reactivity and locomotor activity of the rats suggested a slight effect on the nervous system when compared to controls.
- Body weight:
- No effects were observed.
- Gross pathology:
- All animals appeared normal. No macro- or microscopic abnormalities were detected which could be attributed to the test material.
- Other findings:
- - Organ weights: no changes in lung weights were observed.
- Histopathology: minor changes, not attributable to the test material, were observed.
- Other observations: respiratory frequencies were depressed in the test groups when compared to the controls.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified in accordance with EU Criteria
- Conclusions:
- Under the conditions of the test, the LC50 of the test material was >250 mg/m³. A decreased respiratory frequency, observed during the exposure period, indicates an irritating action of the test material on the respiratory system. Further observations at 1.5 hours after exposure suggest a slight depression of the test material on the behaviour of the treated animals.
- Executive summary:
In a GLP compliant acute inhalation study conducted in line with standardised guidelines EPA OPP 81-3 and partly in line with EU Method B.2, the acute inhalation toxicity of the test material was determined.
Under the conditions of the test the LC50 of the test material in rats was determined to be >250 mg/m3. A decreased respiratory frequency, observed during the exposure period, indicates an irritating action of the test material on the respiratory system. Further observations at 1.5 hours after exposure suggest a slight depression of the test material on the behaviour of the treated animals.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.