5,5'-(1H-isoindole-1,3(2H)-diylidene)dibarbituric acid

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Guideline study, a solubiliser was used

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

no

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution was prepared by mixing 9.2 % test substance with about 20 % HC0 40 and about 70% water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Hardened castor oil = HCO 40

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: common carp
- Source: bred at testing facility. Teichwirtschaftlicher Beispielbetrieb der Bezirksregierung Oberpfalz, Wöllershof, Germany
- Weight at study initiation (mean): 36.3 g


ACCLIMATION
- Type and amount of food: Fukosaim Anschlussfutter 2, Fuko-Kraftfutterfabrik, Ulm, Germany

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Test temperature:

25 ± 2 °C

Dissolved oxygen:

6.8 - 8.3 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Giass vessel (130 l)
- Fill volume: 100 l
- Renewal rate of test solution (frequency/flow rate): 30 l/h per vessel; 720 l per day
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water

Nominal and measured concentrations:

Nominal test substance concentrations:
1 mg/l and 0.1 mg/l
Analytically determined test concentrations [ppm]:
Nominal 1 mg/l: 0.966 (week 2), 0.987 (week 4), 0.966 (week 6), 0.956 (week 8)
Nominal 0.1 mg/l: 0.075 (week 2), 0.076 (week 4), 0.074 (week 6), 0.071 (week 8)

Type:

BCF

Value:

6.3

Remarks on result:

other: mean of two concentration factors measured after 8 weeks of exposure

Remarks:

Conc.in environment / dose:1 mg/l

Type:

BCF

Value:

10.8

Remarks on result:

other: mean of two concentration factors measured after 8 weeks of exposure

Remarks:

Conc.in environment / dose:0.1 mg/l

Details on results:

The acute toxicity was tested in a 48-hour TLm50 study conducted with Oryzias latipes: > 920 and < 1 380 mg active ingredient -dye/l.
No mortality in the bioaccumulation test observed.

Concentration factors in fish.

Nominal test substance concentration	fish	2 weeks	4 weeks	6 weeks	8 weeks
1 mg/l	a	negative	4.14	1.7	10.15
	b	0.42	2.24	3.41	2.44
0.1 mg/l	a	11.40	20.00	21.90	1.20
	b	19.20	negative	0.03	20.40

Reference 2

Endpoint:

bioaccumulation in aquatic species: fish

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

other:

Reason / purpose for cross-reference:

data waiving: supporting information

Description of key information

Accumulation in organisms is not to be expected.

Additional information

Accumulation in organisms is not to be expected.