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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96-Hour LC50 based on nominal test concentrations was greater than 100 mg/l.  The No Observed Effect Concentration was 100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information


A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C.1 of Commission Regulation (EC) No. 440/2008.


Following a preliminary range-finding test fish were exposed, in two groups of seven, to an aqueous solution of the test item, at a single concentration of 100 mg/l for a period of 96 hours at a temperature of approximately 14ºC under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.


The 96-Hour LC50based on nominal test concentrations was greater than 100 mg/l. The No Observed Effect Concentration was 100 mg/l.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

Analysis of the test preparations at 0, 24 and 96 hours showed measured test concentrations to range from 96% to 101% of nominal and so the results are based on nominal test concentrations only.