Trichlorooctylstannane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study followed OECD 202 and EC Guideline 92/69/EEC, but with no GLP information provided.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Principles of method if other than guideline:

Method: other: OECD 202 (1) and EC Guideline 92/69/EEC (2).

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

mono-n-octyltin trichloride

IUPAC Name:

mono-n-octyltin trichloride

Constituent 2

Reference substance name:

ZK 26384

IUPAC Name:

ZK 26384

Details on test material:

- Name of test material (as cited in study report): mono-n-octyltin trichloride
- Analytical purity: 98.86%
- Lot/batch No.: Be 98/44
- Vapour pressure: 0.06 hPa at 25 degrees C
- Safety precautions: routine
- Expiry date/stability: until at least May 1999
- Storage conditions: dry, dark, refrigeration (4 - 6 degrees C)
- Water solubility: 0.33 mg/L at 20 degrees C
- Other: the tin carbon bond of the octyltin compounds is stable in aquous solutions.
- Molecular formula: C8H17Cl3Sn
- Molecular weight: 338.291

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No information.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

No information.

Test solutions

Vehicle:

yes

Details on test solutions:

100 mg of mono-n-octyltin trichloride was dissolved in 1000 mL tap water under constant stirring for 24 hours. Thereafter, the solution was filtered through a glassfibre filter. The filtered solution was used as a test solution. Characteristics of test solution:
- Conductivity: 731 µS/cm
- Hardness (CaCO3): 263 mg/L
- Ca:Mg ratio: 4.4:1

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Parent animals were kept in glass vessels in water of the same quality and temperature as under experimental conditions. The animals were continuously fed with a laboratry culture of microalgae, e.g, Chlorella vulgaris. The Daphnia were exposed for 48 hours under static conditions and were not fed during the test period. The light/dark rhythm was adjusted to 12 hours/12 hours. The Daphnia were between 0 and 24 hours old.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Immobilisation and visual abnormalities were recorded at 24 and 48 hours.

Test conditions

Hardness:

263 mg/L

Test temperature:

20.8-21.6 degrees C. Changes in the temperaure were below the required margin of ± 1 degree C.

pH:

The pH varied between 7.8 at the beginning and 8.5 at the end of the test and was in the rage of 6 - 9 required y the pertinent water quality SOP.

Dissolved oxygen:

The range of dissolved oxygent was between 7.7 and 8.8 mg/L. the oxygen content was above the 60 % saturation limit demanded in the SOP.

Salinity:

No information.

Nominal and measured concentrations:

Nominal concentration was <1 mg/L.

Details on test conditions:

- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Ca/mg ratio: 263 mg/L
- Conductivity: 731 µS/cm
- Intervals of water quality measurement:0 and 48 hours

OTHER TEST CONDITIONS

- Photoperiod: 12 hours light/12 hours dark

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.33 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The hydrographical parameters determined during the exposure were all in the required range. Therefore the quality criteria of th etest were fulfilled. The saturated solution of mono-n-octytin trichloride with the concentration of <1 mg/L (calculated on the basis of TOC analysis) had no adverse effects on the mobility of Daphnia. Thus, it can be concluded that the EC50/48 hours to Daphnia magna is higher than a saturated solution.

Results with reference substance (positive control):

No information.

Reported statistics and error estimates:

No information.

Any other information on results incl. tables

The hydrographical parameters determined during the exposure were all in the required range. Therefore, the quality criteria of the test were fulfilled. The saturated solution of mono-n-octyltin trichloride had no adverse effects on the mobility of Daphnia (Water solubility of the test substance under the test condition was 0.33 mg/L.) The 48 -hr EC50 to Daphnia magna was higher than the concentration of saturated solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No immobilized Daphnia was observed in the test solution. The 48-hr EC50 of Mono-n-octyltin trichloride to Daphnia magna was greater than 0.33 mg/L.

Executive summary:

Short-term toxicity of of mono-n-octyltin trichloride to Daphnia magna was determined in a static aquatic system following OECD 202 and EC Guideline 92/69/EEC. 20 juvenile Daphnia magna were used for the test solution of mono-n-octyltin trichloride and 20 for the tap water control. The Daphnia were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 and 48 hours. The test temperature was in the range of 20.8 to 21.6 ºC, the pH ranged between 7.8 and 8.5, and the oxygen concentration between 7.7 and 8.8 mg/L. The light/dark rhythm was adjusted to12 hours/12 hours. No immobilised Daphnia magna was observed in the test solution at saturation. It was determined that the 48 -hr EC50 was greater than the water solubility of the test substance, which is 0.33 mg/L under the test conditions.