α,α,α-trifluoro-m-toluidine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Remarks:

This study was not conducted in a GLP facility, but was conducted in accordance with the DIN 38412, Part II (Daphnia short-time test) (German Institute of Standardization, 1982).

Data source

Reference

Reference Type:

publication

Title:

Results of the harmful effects of selected water pollutants (anilines, phenols, aliphatic compounds) to Daphnia Magna

Author:

R Kuhn et al.

Year:

1989

Bibliographic source:

Renate Kuhn, Monika Pattard, Klaus-Dieter Pernak and Angeal Winter (1989) Results of the harmful effects of selected water pollutants (anilines, phenols, aliphatic compounds) to Daphnia Magna. Water Research, 23(4): 495-499.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, Part II (Daphnia short-time test) (German Institute of Standardization, 1982)

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-trifluoromethylaniline

IUPAC Name:

3-trifluoromethylaniline

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna from an in-house culture between 6-24 hours old were used for the study.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.4 mmol/L

Test temperature:

20 degrees celsius

pH:

8.0 +/- 0.2

Dissolved oxygen:

The dissolved oxygen content did not fall below 2 mg/L for the duration of the study.

Nominal and measured concentrations:

The actual test concentrations were not reported in the publication, but the following information was provided:
"The concentration steps of the test solution were selected so as to give 3-2 EC values in a range between EC0 and EC100, of which at least one value was below and one above EC50.

Details on test conditions:

The test vessels were two 50 mL beakers containing 20 mL solution per concentration for both the test concentrations and the control. Ten daphnia were added to each vessel.
After a test period of 24 h and 48 h, the number of animals in the control and test solutions that were not immobile were counted. After 48 hours the pH was also measured to determine whether the validity criterion was achieved. The dissolved oxygen concentration was also determined at the end of the study to ensure that the validity criterion was achieved.
The test was considered valid when fewer thatn 10 % of control animals were immobile at the end of the study, when the pH remained above 7.0 and the dissolved oxygen concentration did not fall below 2 mg/L at the end of the study.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

6.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 2.6-17

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 2.0-3.7

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48 hour EC50 of the test item to Daphnia magna is 2.7 mg/L (95% CL: 2.0-3.7).