3,5,8-Trioxa-4-phosphaundec-10-en-1-aminium, 4-hydroxy-N,N,N,10-tetramethyl-9-oxo-, inner salt, 4-oxide

Surface tension

Administrative data

Endpoint:

surface tension

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 November 2008 to 19 March 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

This study was conducted according to the relevant international Guidelines (OECD 115 and OJEC Guideline A.5) using the ring method. The study report provided enough information to clarify the validity of the study and make the methodolgy used wholly reproduceable.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 115 (Surface Tension of Aqueous Solutions)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.5 (Surface Tension)

Deviations:

no

Principles of method if other than guideline:

N/A

GLP compliance:

yes (incl. QA statement)

Remarks:

UK GLP Regulation (1992)

Type of method:

ring method

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

417-560-0

EC Name:

-

Cas Number:

67881-98-5

Molecular formula:

C11H22NO6P

IUPAC Name:

trimethyl[2-({2-[(2-methylprop-2-enoyl)oxy]ethyl phosphonato}oxy)ethyl]azanium

Details on test material:

- Name of test material (as cited in study report): HEMA-PC (2-Methacryloyloxyethyl-2'-(trimethylammoniumethyl) phosphate
- Substance type: Pure Active Substance
- Physical state: Solid
- Analytical purity: 99 % (98-101 %)
- Impurities (identity and concentrations): 0.1 % by area (HPLC)
- Purity test date: 29 September 2008
- Lot/batch No.: SS148076
- Expiration date of the lot/batch: 27 October 2009 (lab default)
- Stability under test conditions: Stable
- Storage condition of test material: Under nitrogen at -20 degrees celsius in the dark.
- Other: N/A

Results and discussion

Surface tension

Surface tension:

72.01 mN/m

Temp.:

20.1 °C

Conc.:

1 g/L

Any other information on results incl. tables

Calibration of Tensiometer

The calibration of the tensiometer with Milli-RO water at a temperature of 24.8°C gave a

mean surface tension value of 71.88 mN/m, with a coefficient of variation of 0.2%. The

literature value of the surface tension of water at 24.8°C is 71.93 mN/m. The correction

factor was therefore determined to be 1.001.

See Table 1.

Measurement of Surface Tension

Triplicate determinations of the surface tension of duplicate aliquots of the test item gave

mean surface tension values of 71.95 and 71.93 mN/m. These values were corrected using

the calibration factor to give surface tension values of 72.02 and 72.00 mN/m.

The overall mean corrected surface tension of HEMA-PC was therefore determined to be

72.01 mN/m.

See Table 2.

Table 1. Calibration of Tensiometer

Temperature (^oC)	Surface Tension (mN/m)	Mean Reading (mN/m)	Coefficient of Variation (%)
24.8	72.06	71.88	0.2
	71.82
	71.77

Table 2. Surface Tension of HEMA-PC

Replicate	Surface Tension (mN/m)	Temperature (^oC)	Mean Surface Tension (mN/m)	Coefficient of Variation (%)	Corrected Surface Tension * (mN/m)	Overall Mean Corrected Surface Tension (mN/m)
A	72.11	20.1	71.95	0.2	72.02	72.01
	71.85
	71.88
B	72.14	20.1	71.93	0.2	72.00
	71.84
	71.82

* Corrected for water calibration factor (1.001)

Applicant's summary and conclusion

Conclusions:

Surface tension was successfully determined for HEMA-PC

Surface Tension = 72.01 mN/m (Coefficient of Variation = 0.2 %)

Executive summary:

Surface tension was successfully determined for HEMA-PC according to OECD Guideline 115.

Surface Tension = 72.01 mN/m (Coefficient of Variation = 0.2 %)