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Partition coefficient

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Reference
Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 2005
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 107 (Partition Coefficient (n-octanol / water), Shake Flask Method)
Version / remarks:
Based on the OECD Test Guideline 107 (Paris, 1981), as published in “Anhang V zur RL 67/548/ EWG”
Principles of method if other than guideline:
Protease activity was measured using an activity test based on the cleavage of Suc-AAPF-pNA (Henkel Biotechnology Method 400.2)
GLP compliance:
no
Type of method:
flask method
Partition coefficient type:
octanol-water
Analytical method:
other: Protease activity was measured using an activity test based on the cleavage of Suc-AAPF-pNA (Henkel Biotechnology Method 400.2)
Key result
Type:
log Pow
Partition coefficient:
-3.1
Temp.:
25 °C
pH:
9.2
Remarks on result:
other: pH of water phase after shaking
Conclusions:
The octanol-water coefficient (logPow) is <= -3.1.
Executive summary:

The partition coefficient was measured based on the OECD Test Guideline 107 (Paris, 1981), as published in “Anhang V zur RL 67/548/ EWG”. After shaking and phase separation, the amount of active protein was determined using an activity test as stated above. In the octanol phase, no activity was detectable. Therefore, the detection limit of the activity test was used as the maximal concentration of active protein present in the octanol phase.

The octanol-water coefficient (logPow) is <= -3.1.

Description of key information

The octanol-water partition coefficient (logPow) for the test substance is <= -3.1 at 25°C.  

Key value for chemical safety assessment

Log Kow (Log Pow):
-3.1
at the temperature of:
25 °C

Additional information

In the octanol phase, no active protein was detectable, therefore, the detection limit of the test method was used as a maximum concentration, giving a log Pow of -3.1.