Tris[2-chloro-1-(chloromethyl)ethyl] phosphate

Eye irritation

Administrative data

Endpoint:

eye irritation, other

Remarks:

in vivo study performed in 1990

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study generated according to internationally accepted testing guidelines and according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Tolgard TDCP MK1
- Physical state: colourless liquid
- Storage condition of test material: In the dark, under ambient conditions
- Analytical purity: not stated

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Cheshire Rabbit Farms Limited, Duddon Lodge, Tarporley, Cheshire.
- Housing: Individually in aluminium cages with grid floor beneath which were peat moss filled trays.
- Diet: ad libitum, standard rabbit diet
- Water: ad libitum
- Acclimation period: 7 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16 - 15 degrees C
- Humidity (%): 56%
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: control eye as only one eye was treated

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 ml
- Concentration (if solution): no data
- Approx. 4 hours before test commencement, both eyes of the rabbits were examined and only aniamls with no ocular defects were used in the test.
- The quantity of material instilled into the treated eye was 0.1 ml
- Instillation of the test material was: placing into the right eye by gently pulling the lower lid away from the eyeball to form a sac into which the test material was dropped. The lids were then gently held together for 1 - 2 seconds.
- The left eye remained untreated and served as control.

Duration of treatment / exposure:

Substance was not washed from eye

Observation period (in vivo):

1, 24, 48, 72h after instillation.

Number of animals or in vitro replicates:

3 animals (two male and one female).

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): not done

SCORING SYSTEM: OECD recommended scoring system

TOOL USED TO ASSESS SCORE: 420/5.5x Panoramic Loupe and pen torch (Pifco).

Results and discussion

In vivo

Results

Irritation parameter:

conjunctivae score

Remarks:

slight conjunctival erythema observed

Basis:

mean

Time point:

other: 1h

Score:

> 0 - <= 1

Max. score:

1

Reversibility:

fully reversible within: 24h

Remarks on result:

other: Conjunctival erythema (score 1) was observed in all 3 animals at 1h, along with slight discharge, all were reversed by 24 h.

Irritant / corrosive response data:

Test substance caused slight erythema, is slightly irritating but under the given experimental conditions would not be considered as corrosive.

Applicant's summary and conclusion

Interpretation of results:

slightly irritating

Remarks:

Migrated information

Conclusions:

Under the given experimental conditions TDCP is slightly irritating, but not corrosive and quickly reversible in New Zealand White rabbits.