2,2,6,6-tetramethyl-4-piperidone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study according to guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,6,6-tetramethyl-4-piperidone

EC Number:

212-554-2

EC Name:

2,2,6,6-tetramethyl-4-piperidone

Cas Number:

826-36-8

Molecular formula:

C9H17NO

IUPAC Name:

2,2,6,6-tetramethylpiperidin-4-one

Details on test material:

- Name of test material (as cited in study report): Triacetonamin, TAA
- Physical state: yellow-brown paste
- Analytical purity: 69.9%
- Lot/batch No.: Pt 13800

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Strain: IRCHA
- Source: in-house breed
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: females kept in beakers filled with M4-medium according to Elendt (1990)
- Feeding during breeding:
- Food type: Scenedesmus subspicatus
- Amount: as required
- Frequency: daily
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol (sum of Ca2+ and Mg2+)

Test temperature:

20 °C +/- 1°C

pH:

7.8-8.6

Dissolved oxygen:

8.1-8.3 mg/L

Salinity:

no data

Nominal and measured concentrations:

0, 110, 160, 220, 320, 450, 650 and 900 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, fill volume: glas vessels with 10 mL volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2 mL per test organism

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater
- Chlorine: CaCl2 x H2O 294 mg/L, KCl 5.5 mg/L
- Ca/Mg ratio: 4:1

OTHER TEST CONDITIONS
- Photoperiod: dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

160 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

281.2 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Remarks on result:

other: 246.1 - 321.3 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

650 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Results with reference substance (positive control):

40% immobility at 0.9 mg/L, 100% immobility at 1.9 mg/L after 24 h exposure to potassium dichromate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of this daphnia immobilization study the test item triactonamine showed an EC50 of 281.2 mg/L (95% CL: 246.1-321.3 mg/L).