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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study with acceptable restrictions from analogeous substance

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: German Industrial Standard DIN 38412 part 15
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Sarcosine
EC Number:
203-538-6
EC Name:
Sarcosine
Cas Number:
107-97-1
Molecular formula:
C3H7NO2
IUPAC Name:
N-methylglycine
Details on test material:
IUCLID4 Test substance: other TS

TS-Freetext:
- CHEMICAL NAME: sarcosin
- CAS No.: 107-97-1
- PURITY: 99 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: golden orfe
- Source: Fischzucht Pau Eggers, Hohenweststedt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.1 - 6.4 cm
- Weight at study initiation (mean and range, SD): 1.8 - 3.3 g
- Feeding during test: none


ACCLIMATION
- Acclimation period: 2 weeks
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Growing feed F/B 50, ad libitum
- Health during acclimation (any mortality observed): none
- mortality during housing: ca. 0.1%

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 10 l volume
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to test guideline
- Ca/mg ratio: 4:1
- Conductivity: max. 10 micro MHO

OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16:8

Reference substance (positive control):
yes
Remarks:
Chloracetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:
- biological observations: no symptoms at any concentration
- cumulative mortality: no mortality at any concentration
- reference substances: positive control of animals conducted with chloroacetamide; LC 50 after 48 hours: about 32 mg/L (the lethal concentration corresponds to normal sensitivity)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes