Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 225-383-3 | CAS number: 4813-57-4
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
In the toxicity to microorganisms test the EC50 value of the test
substance, 2-Propenoic acid, C16-18-alkyl esters determined to be
greater than 1000 mg/L.
The EC50 value for 2 -Proenoic aicd, C18-26-alkyl ester was determined to be greater than 1000 mg/L and also the EC10 was determined to be greater than 1000 mg/L.
Based on this result the EC50 value of 2-Propenoic acid, octadecyl ester is also considered to be >1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The objective of this key study according to OECD No. 209 was to determine the effect of different concentrations of the test substance, 2-Propenoic acid, C16-18-alkyl esters on the oxygen cossumption of microbial-inoculum, compared to the blank control. The test was conducted at concentrations of 62.5, 125, 250, 500 and 1000 mg/L. Based on these results, the EC50 value was calculated to be greater than 1000 mg/L.
The EC50 value of for the reference substance 3,5-dichlorophenol was calculated to be 7.4 mg/L, which was within the acceptable limits, (5.0 to 30 mg/L) as specified in OECD 209 Guideline. Thus, all validation criteria were fulfilled.
A determination of the Inhibition of oxygen consumption in the activated sludge respiration Inhibition test for the the read across test substance 2 -Propenoic acid, C18 -26 -alkyl ester was performed. The study was conducted according to the following test guidelines: OECD Guideline 209. The test was conducted at concentrations 1000, 500, 250, 125, 62.5 mg/L as nominal concentration based on test substance without correction of purity or composition and blank controls. Incubation time was 3 hours. Sludge concentration in the test: 1.5 g/L and the inoculum was Activated sludge from a municipal wastewater treatment plant from the municipal wastewater treatment plant of Mannheim, Germany.
Based on these results, the EC50 value was calculated to be greater than 1000 mg/L.
The EC50 value of for the reference substance 3,5-dichlorophenol was calculated to be 5.3 mg/L, which was within the acceptable limits (5.0 to 30 mg/L) as specified in OECD 209 Guideline. Thus, all validation criteria were fulfilled.
Based on this result the EC50 value of 2-Propenoic acid, octadecyl ester is also considered to be >1000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
