Registration Dossier
Registration Dossier
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EC number: 203-401-0 | CAS number: 106-47-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles
Data source
Reference
- Reference Type:
- publication
- Title:
- Testing for the toxicity of chemicals with Tetrahymena pyriformis
- Author:
- Yoshioka Y, Ose Y, and Sato T
- Year:
- 1 985
- Bibliographic source:
- Sci. Total Environ. 43, 149-157
Materials and methods
- Principles of method if other than guideline:
- other: cell multiplication inhibition test
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-chloroaniline
- EC Number:
- 203-401-0
- EC Name:
- 4-chloroaniline
- Cas Number:
- 106-47-8
- Molecular formula:
- C6H6ClN
- IUPAC Name:
- 4-chloroaniline
- Details on test material:
- - Name of test material (as cited in study report): p-Chloroaniline
- Analytical purity: "analytical grade"
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- - Stock solution of test substance was added to the sterile medium to provide a concentration ratio
of 1.8 in 10 ml of 2 % proteose peptone.
- The solutions were then inoculated with 0.2 ml of the pre-cultured T. pyriformis and cultivated at
a temperature of 30 °C for 24 h without agitation.
Test organisms
- Test organisms (species):
- Tetrahymena pyriformis
- Details on inoculum:
- - Tetrahymena pyriformis was preserved in a sterile medium (of 2 % proteose peptone at a temperature of 20 °C) which was renewed at 2-4 week intervals.
- Tetrahymena pyriformis was pre-cultured at a temperature of 30 °C for 24 h.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Test temperature:
- 30 °C
Results and discussion
Effect concentrations
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Applicant's summary and conclusion
- Executive summary:
A cell-multiplication-inhibition test on the acute toxicity of 4-chloroaniline to Tetrahymena pyriformis was performed for a test period of 24 hours. An EC50 value of 10 mg/l was reported (Yoshioka et al., 1985).
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