Sodium sulphanilate

Additional toxicological data

Administrative data

Endpoint:

additional toxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

September 20, 1988

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Experimental result from a Bibliographic source.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Deviations:

not applicable

Test material

Test material information

Constituent 1

Reference substance name:

Sodium sulphanilate

EC Number:

208-208-5

EC Name:

Sodium sulphanilate

Cas Number:

515-74-2

Molecular formula:

C6H7NO3S.Na

IUPAC Name:

sodium 4-aminobenzenesulfonate

Results and discussion

Any other information on results incl. tables

The relative toxicity of the test compounds was determined by use of the PI₅₀, which is the concentration of test compound required to induce a 50% reduction in cell protein content.

Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI₅₀value. Surfactants and heavy metals both have very low PI₅₀values. In all other groups a gradually increasing series is seen from low to high PIs0 values.

Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI₅₀(Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM.

Applicant's summary and conclusion

Conclusions:

Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50 value. Surfactants and heavy metals both have very low PI50 values. In all other groups a gradually increasing series is seen from low to high PIs0 values.


Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI50 value. Surfactants and heavy metals both have very low PI50 values. In all other groups a gradually increasing series is seen from low to high PIs0 values.

Citotoxicity of 114 compounds has been assessed as an screening assay intended to produce information of the toxicity effect of the test substances. The results suggest that the assay could be a useful pre-screening method to test for the cytotoxicity of chemicals.Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI50 (Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM.

Executive summary:

Supporting study:

An increasing demand for toxicity evaluation together with an increasing resistance to experiments using laboratory animals have led to the need for in vitro testing systems. Cytotoxicity studies can provide information about the potency and mechanism of action of xenobiotics, usually more rapidly and at lower cost than in vivo studies.

With the screening assay it was not intended to produce detailed information on mechanisms of toxicity, but rather to show a general biological effect that occurs after the living cells have come into contact with the test chemical.

The cellular protein content measured in cultured Hep G2 cells was used as the endpoint for determining the cytotoxicity of a range of 114 chemical compounds. The relative toxicity of the test compounds was quantified by the determination of the PI₅₀, which is the concentration of xenobiotic required to produce a 50% reduction in protein content of the culture after 24 hours.

Surfactants and heavy metals consistently had low PIs0 values. Hep G2 cells were very sensitive to compounds with more than one carboxyl group. Triacetin and glutathione were identified as false positives. The results suggest that the PI₅₀ assay could be a useful pre-screening method to test for the cytotoxicity of chemicals.

Results for 114 compounds are summarized in this publication. The compounds belong to many different chemical classes, and were presented in 7 groups. Within each group the compounds were arranged according to increasing PI₅₀value. Surfactants and heavy metals both have very low PI₅₀values. In all other groups a gradually increasing series is seen from low to high PIs0 values.

Citotoxicity related to sodium 4-aminobenzenesulfonate in terms of PI₅₀(Concentration of test compound that induces a 50% decrease in cell proteins, determined after 24 hours of treatment) was calculated to be 97 mM.