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EC number: 243-001-3 | CAS number: 19372-44-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 October 1991 - 05 November 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was performed according to OECD guideline and GLP. No CoA included in the report. One of the strains: E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102 was not included in the study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- - One of the strains: E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102 was not included in the study.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Bis(pentane-2,4-dionato)calcium
- EC Number:
- 243-001-3
- EC Name:
- Bis(pentane-2,4-dionato)calcium
- Cas Number:
- 19372-44-2
- Molecular formula:
- C10H14CaO4
- IUPAC Name:
- calcium bis[(2Z)-4-oxopent-2-en-2-olate]
- Details on test material:
- Name: Ca-Acetylacetonate
Batch No.: 106002
Physical state: Powder
Purity: 98%
No CoA included in the report.
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- 11.9 µg; 59.5 µg; 119.0 µg; 595.0 µg and 1190.0 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracene
- Remarks:
- Details see below
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: NA
- Exposure duration: 48 hours
SELECTION AGENT (mutation assays): histidine
NUMBER OF REPLICATIONS: triplicate colonies, duplicate experiment
NUMBER OF CELLS EVALUATED: the plates were scored by counting the number of revertant colonies on each plate.
DETERMINATION OF CYTOTOXICITY
- Method: bacterial background growth - Evaluation criteria:
- The revertant colonies on each plate were counted. From each of 3 plates at each dose level the mean values together with standard deviations and enhancement factors as compared to the spontaneous reversion rates were determined.
A test article is considered as mutagenic, if either a significant dose-related increase in the number of revertants or a significant and reproducible increase for at least one test concentration is induced.
A test article producing neither a significant dose-related increase in the number of revertants nor a significant and reproducible positive response at anyone of the test points is considered non-mutagenic in this system.
A significant response is described as follows:
A test article is considered as mutagenic if in strain TA 100 the number of revertants will be at least twice as high and in strains TA 98, TA 1535 und TA 1537 it will be at least three times higher as compared to the spontaneous
reversion rate.
Also, a dose-dependent increase in the number of revertants is regarded as an indication of possibly existing mutagenic potential of the test article regardless whether the highest dose will induce the above described enhancement factors or not.
The generally accepted conditions for the evalutation of the results will be: corresponding background growth on both negative control and test plates normal range of spontaneous reversion rates referred to the negative control groups without metabolic activation
Range of spontaneous reversion frequencies:
TA 98 15-60
TA 100 75-200
TA 1535 3-37
TA1537 4-31
These values refer to the negative control group without metabolic activation. - Statistics:
- None
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test article did not induce point mutations by base pair changes or frameshifts in the genome of the strains used.
Therefore, calcium acetylacetonate is considered to be non-mutagenic in this Salmonella typhimurium reverse mutation assay. - Executive summary:
This study was performed to investigate the potential of calcium acetylacetonate to induce gene mutations according to the plate incorporation test using Salmonella typhimurium strains TA 98, TA-100, TA 1535 and TA 1537.
The assay was performed in two independent experiments using identical procedures, both with and without liver microsomal activation. Each
concentration, including the controls, was tested in triplicate. Calcium acetylacetonate was tested at the following concentrations: 11.9 µg; 59.5 µg; 119.0 µg; 595.0 µg and 1190.0 µg/plate
Precipitates were neither observed in the whole concentration range nor in the overlay agar. The plates incubated with the test article showed normal background growth up to 1190.0 µg/plate with and without S9-mix in all strains used. Up to the highest investigated dose, neither a significant and reproducible increase in the number of revertants was found in any strain as compared to the solvent control nor a concentration dependent enhancement of the revertant number exists. The presence of liver microsomal activation did not influence these findings. Appropriate reference mutagens were used as positive controls and showed a distinct increase in induced revertant colonies.
In conclusion, it can be stated that during the described mutagenicity test and under the experimental conditions reported, the test article did not induce point mutations by base pair changes or frameshifts in the genome of the strains used. Therefore, calcium acetylacetonate is considered to be non-mutagenic in this Salmonella typhimurium reverse mutation assay.
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