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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
non GLP, no analytical concentration verification

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
4,4'-diaminostilbene-2,2'-disulphonic acid
EC Number:
201-325-2
EC Name:
4,4'-diaminostilbene-2,2'-disulphonic acid
Cas Number:
81-11-8
Molecular formula:
C14H14N2O6S2
IUPAC Name:
2,2'-ethene-1,2-diylbis(5-aminobenzenesulfonic acid)
Details on test material:
- Name of test material (as cited in study report): 4,4'-diaminostilbene-2,2'-disulfonic acid
- Analytical purity: no data
Radiolabelling:
not specified

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g
- Lipid content of fish: 3.5 %

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
6 wk

Test conditions

Test temperature:
25 ± 2 °C
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volum = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 ml/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week
Nominal and measured concentrations:
Nominal: 0.1 and 1.0 mg/L

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
< 0.5 dimensionless
Basis:
not specified
Remarks on result:
other: Conc.in environment / dose:1.0 mg/l
Type:
BCF
Value:
< 4.7 dimensionless
Basis:
not specified
Remarks on result:
other: Conc.in environment / dose:0.1 mg/l

Applicant's summary and conclusion

Conclusions:
BCF < 0.5 (dimensionless)
BCF < 4.7 (dimensionless)
Executive summary:

BCF < 0.5 (dimensionless)
BCF < 4.7 (dimensionless)