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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Endpoint summary

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Administrative data

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Ames test (OECD471): not mutagenic

Additional information

An Ames test in accordance with OECD471 was performed to determine the mutagenic potential of cinnamon leaf oil to cause gene mutations in bacteria. Cinnamon Leaf Oil did not induce a significant dose-related increase in the number of revertant (His+) colonies in each of the four tester strains (TA1535, TA1537, TA98 and TA100) and in the number of revertant (Trp+) colonies in test strain WP2uvrA both in the absence and presence of S9-metabolic activation. These results were confirmed in an independently repeated experiment. Furthermore, the negative and strain-specific positive control values were within the laboratory historical control data ranges indicating that the test conditions were adequate and that the metabolic activation system functioned properly. Based on the results of this study it is concluded that Cinnamon Leaf Oil is not mutagenic in the Salmonella typhimurium reverse mutation assay and in the Escherichia coli reverse mutation assay.

An additional study is included that was disregarded. In this study, cinnamon leaf oil was tested for mutagenicity in the Ames test without metabolic activation in four S. typhimurium strains (TA98, TA1535, TA1537 and TA1538) at two concentrations (5 and 10 pl/plate). Cinnamon leaf exhibited mutagenicity in the Ames test at the lowest dose (5 pl/plate) in two of the four strains tested, i.e. S. typhimurium TA98 (weak mutagen – colonies more than control) and TA1537 (mutagen - colonies more than 70% of control). As the effects were observed at the lowest dose only, and no independent repeat was performed, no conclusion can be drawn based on this study.

Based on the findings in the key study, in which six doses were tested, with and without metabolic activation, and an independent repeat was performed, it can be concluded that cinnamon leaf oil does not cause gene mutations in bacteria.

Short description of key information:
In vitro gene mutation in bacteria: not mutagenic (OECD471)

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Based on the available information for Cinnamon leaf oil, the substance can be considered not mutagenic and should therefore not need to be classified for mutagenicity according to the criteria outlined in Annex I of the CLP Regulation (1272/2008/EC). However, based on the Safrole content in Cinnamon leaf oil the substance does need to be classified as genotoxic.