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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1992-11-23 until 1992-11-27
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not enough details to assign a reliability rating of 1

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
DIN 38412 part 15
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
Dodecylphenol
EC Number:
248-312-8
EC Name:
Dodecylphenol
Cas Number:
27193-86-8
IUPAC Name:
2-dodecylphenol
Constituent 2
Chemical structure
Reference substance name:
Phenol, dodecyl-, branched
EC Number:
310-154-3
EC Name:
Phenol, dodecyl-, branched
Cas Number:
121158-58-5
Molecular formula:
C18H30O
IUPAC Name:
4-(3,4,5-trimethylheptyl)phenol
Details on test material:
- Name of test material (as cited in study report): Dodecylphenol T
- Substance type: standard sample
- Physical state: liquid
- Analytical purity: 99.9%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Lot/batch No.: 3633/81416
- Date of production: 1992-05-07
- Stability under test conditions: 12 month
- Storage condition of test material: under nitrogen

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
determination after 0, 24, 48 and 72 h via HPLC

Test solutions

Vehicle:
no
Details on test solutions:
saturated solution, act of process not described.

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Strain: Leuciscus idus melanotus
- Source: Fischzucht Eggers, Hohenwestedt, Germany
- Age at study initiation (mean and range, SD): not mentioned
- Length at study initiation (length definition, mean, range and SD): 6 ± 2 cm, no further details mentioned
- Weight at study initiation (mean and range, SD): not mentioned
- Method of breeding: not mentioned
- Feeding during test: none

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): The fish were kept in 200 l earthenware basins in flow rate.
- Pretreatment: The fish were attended with ZEPHIROL 1:50 000 for 1 hour and held in a 14 day quarantine. The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.
- Type and amount of food: TetraMin, administration in an amount of approx. 3 % of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
none

Test conditions

Hardness:
approx. 11.4 °dH
Test temperature:
20 ± 1 °C
pH:
8.1 - 8.4
Dissolved oxygen:
8.9 - 9.4 mg/l
Salinity:
not mentioned
Nominal and measured concentrations:
Nominal: 1.6 and 2.0 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): not mentioned
- Material, size, headspace, fill volume: 20 l, 10 l fill volume, no further details mentioned
- Aeration: continously, no further details mentioned
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not mentioned

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking water, no further details mentioned
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: not mentioned

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality; pH, temperature, O2 saturation

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned, saturated solution
- Range finding study: not mentioned
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
other: > satureated solution
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
saturated solution
Basis for effect:
mortality (fish)
Results with reference substance (positive control):
no reference substance tested
Reported statistics and error estimates:
no statisitcs performed

Any other information on results incl. tables

Sublethal observations / clinical signs:

 concentration [mg/L]  No. of fish at start of study
     96 h
    number   mortality[%]
saturated solution  10 0 0
control  10 0 0

Applicant's summary and conclusion

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