Phenol, dodecyl-, branched

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-11-23 until 1992-11-27

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Not enough details to assign a reliability rating of 1

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

DIN 38412 part 15

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dodecylphenol

EC Number:

248-312-8

EC Name:

Dodecylphenol

Cas Number:

27193-86-8

IUPAC Name:

2-dodecylphenol

Constituent 2

Reference substance name:

Phenol, dodecyl-, branched

EC Number:

310-154-3

EC Name:

Phenol, dodecyl-, branched

Cas Number:

121158-58-5

Molecular formula:

C18H30O

IUPAC Name:

4-(3,4,5,6-tetramethyloctan-2-yl)phenol

Details on test material:

- Name of test material (as cited in study report): Dodecylphenol T
- Substance type: standard sample
- Physical state: liquid
- Analytical purity: 99.9%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Lot/batch No.: 3633/81416
- Date of production: 1992-05-07
- Stability under test conditions: 12 month
- Storage condition of test material: under nitrogen

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

determination after 0, 24, 48 and 72 h via HPLC

Test solutions

Vehicle:

no

Details on test solutions:

saturated solution, act of process not described.

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Strain: Leuciscus idus melanotus
- Source: Fischzucht Eggers, Hohenwestedt, Germany
- Age at study initiation (mean and range, SD): not mentioned
- Length at study initiation (length definition, mean, range and SD): 6 ± 2 cm, no further details mentioned
- Weight at study initiation (mean and range, SD): not mentioned
- Method of breeding: not mentioned
- Feeding during test: none

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): The fish were kept in 200 l earthenware basins in flow rate.
- Pretreatment: The fish were attended with ZEPHIROL 1:50 000 for 1 hour and held in a 14 day quarantine. The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.
- Type and amount of food: TetraMin, administration in an amount of approx. 3 % of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The animals showed a normal behaviour pattern at the beginning of the test and were free from visible diseases.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

approx. 11.4 °dH

Test temperature:

20 ± 1 °C

pH:

8.1 - 8.4

Dissolved oxygen:

8.9 - 9.4 mg/l

Salinity:

not mentioned

Nominal and measured concentrations:

Nominal: 1.6 and 2.0 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): not mentioned
- Material, size, headspace, fill volume: 20 l, 10 l fill volume, no further details mentioned
- Aeration: continously, no further details mentioned
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: not mentioned

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated drinking water, no further details mentioned
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: not mentioned

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality; pH, temperature, O2 saturation

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned, saturated solution
- Range finding study: not mentioned

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

other: > satureated solution

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

saturated solution

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

no reference substance tested

Reported statistics and error estimates:

no statisitcs performed

Any other information on results incl. tables

Sublethal observations / clinical signs:

concentration [mg/L]	No. of fish at start of study
		96 h
		number	mortality[%]
saturated solution	10	0	0
control	10	0	0