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EC number: 310-154-3 | CAS number: 121158-58-5
Ecotoxicological information
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-04-19 until 1995-04-21
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phenol, dodecyl-, branched
- EC Number:
- 310-154-3
- EC Name:
- Phenol, dodecyl-, branched
- Cas Number:
- 121158-58-5
- Molecular formula:
- C18H30O
- IUPAC Name:
- 4-(3,4,5,6-tetramethyloctan-2-yl)phenol
- Reference substance name:
- Dodecylphenol
- EC Number:
- 248-312-8
- EC Name:
- Dodecylphenol
- Cas Number:
- 27193-86-8
- IUPAC Name:
- 2-dodecylphenol
- Details on test material:
- - Name of test material (as cited in study report): Dodecylphenol T
- Substance type: technical product
- Physical state: liquid
- Analytical purity: 100%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Purity test date: not mentioned
- Lot/batch No.: continuously production; 3630/81325
- Date of production: 1991-04-25
- Stability under test conditions: 1 year
- Storage condition of test material: not mentioned
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- - Dispersion: no
- Vehicle, solvent: acetone
- Concentration of stock solution: 1 g/l
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, IRCHA
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 l beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of 4-5 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.
- Feeding during test: none
ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- not mentioned
- Test temperature:
- 20 °C ± 1 °C
- pH:
- 7.4 - 7.5
- Dissolved oxygen:
- 7.8 - 8.1 mg/l
- Salinity:
- CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l
Ca/Mg ratio: 4:1
Na/K ratio: 10:1 - Nominal and measured concentrations:
- nominal concentration: 0 (control) / 20 / 28 / 40 / 56 / 80 / 112 / 160 / 220 / 320 / 450 µg/l and acetone 450 µl/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l)
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Conductivity: not mentioned
- Culture medium different from test medium: yes, M4 medium, Elendt
- Intervals of water quality measurement: not mentioned
OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours; O2 concentration and pH 0 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 106 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 92.7 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Remarks on result:
- other: 95% CL: 81.1 - 105.9 µg/L
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 56 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 160 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Concentrations: 0.9 and 1.9 mg/l potassium dichromate
- Results: 50% at 0.9 mg/l and 100% at 1.9 mg/l immobilised daphnia after 24 h - Reported statistics and error estimates:
- The EC50 (48h) value was calculated with Propit-analysis according to Cavalli-Sforza (1972), the EC50 (24h) was calculated graphically (dose-response relationship).
Any other information on results incl. tables
Table #1: Effect of o-tert.-Butylphenol on immobilisation of Daphnia magna
| concentration[µg/L] | 24 hour values | 48 hour values | ||||
| number | % | number | % | |||
| mobile | immobile | immobile | mobile | immobile | immobile | |
| 0 (control) | 20 | 0 | 0 | 20 | 0 | 0 |
| 20 | 20 | 0 | 0 | 20 | 0 | 0 |
| 28 | 20 | 0 | 0 | 20 | 0 | 0 |
| 40 | 20 | 0 | 0 | 20 | 0 | 0 |
| 56 | 20 | 0 | 0 | 19 | 1 | 5 |
| 80 | 16 | 4 | 20 | 15 | 5 | 25 |
| 112 | 7 | 13 | 65 | 5 | 15 | 75 |
| 160 * | 0 | 19 | 100 | 0 | 19 | 100 |
| 220 | 0 | 20 | 100 | 0 | 20 | 100 |
| 320 | 0 | 20 | 100 | 0 | 20 | 100 |
| 450 | 0 | 20 | 100 | 0 | 20 | 100 |
| acetone | 20 | 0 | 100 | 20 | 0 | 0 |
* 19 daphnia deployed
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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