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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1995-04-19 until 1995-04-21
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Phenol, dodecyl-, branched
EC Number:
310-154-3
EC Name:
Phenol, dodecyl-, branched
Cas Number:
121158-58-5
Molecular formula:
C18H30O
IUPAC Name:
4-(3,4,5-trimethylheptyl)phenol
Constituent 2
Reference substance name:
Dodecylphenol
EC Number:
248-312-8
EC Name:
Dodecylphenol
Cas Number:
27193-86-8
IUPAC Name:
2-dodecylphenol
Details on test material:
- Name of test material (as cited in study report): Dodecylphenol T
- Substance type: technical product
- Physical state: liquid
- Analytical purity: 100%
- Impurities (identity and concentrations): not mentioned
- Composition of test material, percentage of components: not mentioned
- Purity test date: not mentioned
- Lot/batch No.: continuously production; 3630/81325
- Date of production: 1991-04-25
- Stability under test conditions: 1 year
- Storage condition of test material: not mentioned

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
- Dispersion: no
- Vehicle, solvent: acetone
- Concentration of stock solution: 1 g/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: Daphnia magna Straus, IRCHA
- Source: laboratory breed
- Age at study initiation: not older than 24 hours
- Method of breeding: The females were maintained in M4 medium in 1 l beakers. A reddish dark-brown coloration of the animals indicated that they were healthy. At two to three day intervals, the exuviae were siphoned off and the water was changed. In the course of this, the offspring was removed from the breeding vessels. At regular intervals of 4-5 weeks, offspring was isolated from the vessels. They served as a basis for further breeding.
- Feeding during test: none

ACCLIMATION
- Acclimation period: Females were seperated from the offspring 24 hours before the start of the test. The offspring produced overnight were transferred into test water and were used for the test.
- Type and amount of food: Scenedesmus subspicatus, the amount of food added is such that it is consumed by the animals
- Feeding frequency: daily

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
not mentioned
Test temperature:
20 °C ± 1 °C
pH:
7.4 - 7.5
Dissolved oxygen:
7.8 - 8.1 mg/l
Salinity:
CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l
Ca/Mg ratio: 4:1
Na/K ratio: 10:1
Nominal and measured concentrations:
nominal concentration: 0 (control) / 20 / 28 / 40 / 56 / 80 / 112 / 160 / 220 / 320 / 450 µg/l and acetone 450 µl/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Round-bottom test tubes
- Material, size, headspace, fill volume: glass, calibrated to 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l)
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Conductivity: not mentioned
- Culture medium different from test medium: yes, M4 medium, Elendt
- Intervals of water quality measurement: not mentioned

OTHER TEST CONDITIONS
- Adjustment of pH: not adjusted
- Photoperiod: not applicable
- Light intensity: no light during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation after 24 and 48 hours; O2 concentration and pH 0 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not mentioned
- Range finding study: not mentioned
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
106 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: immobilisation
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
92.7 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: immobilisation
Remarks on result:
other: 95% CL: 81.1 - 105.9 µg/L
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
56 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: immobilisation
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
160 µg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: immobilisation
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Concentrations: 0.9 and 1.9 mg/l potassium dichromate
- Results: 50% at 0.9 mg/l and 100% at 1.9 mg/l immobilised daphnia after 24 h
Reported statistics and error estimates:
The EC50 (48h) value was calculated with Propit-analysis according to Cavalli-Sforza (1972), the EC50 (24h) was calculated graphically (dose-response relationship).

Any other information on results incl. tables

Table #1: Effect of o-tert.-Butylphenol on immobilisation of Daphnia magna

  concentration[µg/L]        24 hour values        48 hour values
      number  %  number   
   mobile  immobile  immobile  mobile  immobile immobile 
 0 (control) 20 0 0 20 0 0
20 20 0 0 20 0 0
28 20 0 0 20 0 0
40 20 0 0 20 0 0
56 20 0 0 19 1 5
80 16 4 20 15 5 25
112 7 13 65 5 15 75
160 * 0 19 100 0 19 100
220 0 20 100 0 20 100
320 0 20 100 0 20 100
450 0 20 100 0 20 100
 acetone 20 0 100 20 0 0

* 19 daphnia deployed

Applicant's summary and conclusion

Validity criteria fulfilled:
yes

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