Registration Dossier
Registration Dossier
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EC number: 200-539-3 | CAS number: 62-53-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Depending on local conditions and existing concentrations, disturbances in the biodegradation process of activated sludge are possible
NOEC = 2 mg/l (nitrification inhibition)
Key value for chemical safety assessment
Additional information
Several single species tests and activated sludge tests are available for assessing the toxicity of Aniline to microorganisms. APNECmicroorganismderived from the most relevant test resulting in the lowest PNEC value is used for assessment. In order to protect the efficiency of domestic and industrial waste water treatment plants, inhibition of nitrification is identified to be the most sensitive test system.Nitrification inhibition was measured on both, activated sludge (Bayer AG 2000) and on the strain Nitrosomonas sp. (Hockenbury and Grady 1977), respectively. Nitrification inhibition on activated sludge was determined according to ISO DIS 9509 (Method for Assessing the Inhibition of Nitrification of Activated Sludge Microorganisms by Chemicals and Waste Waters). An EC50 of 7 mg/l and and a NOEC of 2 mg/L was determined after 2 hours incubation (Bayer AG 2000). Nitrosomonas sp., however, exhibited an EC50 of <1 mg/l (Hockenbury and Grady 1977). In contrast to the low effect concentrations as observed in nitrification experiments, respiration inhibition was only observed at very high substance concentrations (EC20 = 2800 mg/L, BASF AG 1979). The results with the ciliated protozoa show lower effect values as tests using activated sludge as inoculum.For these kind of studies, an assessment factor of 1 has to be applied for the derivation of PNECmicroorganisms., resulting in PNECmicroorganism between 2.4 and 25 mg/l.For the risk assessment of sewage treatement plants, the PNECmicroorganism was derived from the nitrification inhibition test on activated sludge (NOEC = 2 mg/l), using an assessment factor of 1, resulting in the lowest PNEC value.
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