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EC number: 701-024-0 | CAS number: 26038-87-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- During the final test the temperature was stable within 2.3 instead of 2.0ºC. Evaluation: Extend of the deviation was insignificant and therefore, had no effect on the results.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method:
- Sample storage conditions before analysis: - Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Reference substance (positive control):
- yes
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 67 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 52 - 85 mg a.i./L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL 21 - 47 mg a.i./L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of a GLP OECD 201 study with Pseudokirchneriella subcapitata, MEA Polyborate 1:3 reduced growth rate and inhibited the yield of this fresh water algae species significantly at 32 mg a.i./l and higher.
The EC50 for growth rate reduction (ERC50: 0-72h) was 67 mg a.i./l with a 95 % confidence interval ranging from 52 to 85 mg a.i./l.
The EC50 for yield inhibition (EYC50: 0-72h) was 32 mg a.i./l with a 95 % confidence interval ranging from 21 to 47 mg a.i./l.
The NOEC for growth rate reduction and yield inhibition was 18 mg a.i./l.
- Executive summary:
Pseudokirchneriella subcapitata, Fresh Water Algal Growth Inhibition Test with
MEA Polyborate 1:3.
The study procedures described in this report were based on the OECD guideline No. 201, 2006. In addition, the procedures were designed to meet the test methods of the Commission Regulation (EC) No 440/2008, Part C.3, 2008; Amended by EC No. 761/2009and the ISO International Standard 8692, 2004.
The test substance, MEA Polyborate 1:3, is a complex multi-component substance (a UVCB or unknown variable complex or biological) substance containing salt and non-salt compounds in rapid equilibrium. The test substance is prepared from monoethanolamine, borate compounds and water. The non-water components (regarded as the active ingredient, a.i.) are 68% (w/w) of the test substance. The batch of test substance used was a clear colourless liquid and completely soluble in test medium at the concentrations tested.
A final test was performed based on the results of a range-finding test. Preparation started with a concentration of 100 mg active ingredient (a.i.)/l which corresponds to 147 mg MEA Polyborate 1:3 per litre. To this end 115 µl of the test substance was added to 1000 ml test medium and magnetically stirred for a period of 15 minutes. The pH of the highest concentration was adjusted with 1M HCl (Merck, Darmstad, Germany). The lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium.
Samples taken from the nominal concentrations of 14.7, 26.5, 47, 82 and 147 mg/l (i.e. 10, 18, 32, 56 and 100 mg a.i./l) were analysed. The concentrations of both boron and MEA were determined.
Boron analyses: The nominal concentrations of boron in analysed samples were 1.71, 3.07, 5.45, 9.55 and 17.1 mg boron per litre, respectively.
Analysis of the samples taken at the start of the final test showed that the actual concentrations were in agreement with nominal (90 - 92% of nominal) and except for the samples taken from the nominal concentrations of 18 and 56 mg a.i./l, the measured concentrations remained stable during the entire period of exposure (99 – 107% of initial). The measured concentrations in the samples taken from nominally 18 and 56 mg a.i./l decreased to 21 and 54% of initial after 24 hours of exposure, respectively. The measured concentrations remained stable to the end of the exposure.
MEA analyses: The nominal concentrations of MEA in analysed samples were 2.5, 4.51, 7.99, 14 and 25 mg MEA per litre, respectively.
Analysis of the samples taken at the start of the final test showed that the actual concentrations were in agreement with nominal, i.e 99 - 107% of nominal and remained stable during the entire test period (86 - 99% of initial).
The cause of the decrease of boron concentrations in two samples during the first 24 hours of exposure is not known. Note that at the same time the concentration of MEA in the same test solutions remained stable. Moreover, the concentrations of boron in the mentioned samples did not decrease during the following 48 hours of exposure and in all other test groups the concentrations of boron were stable during the entire test period. Therefore, the effect concentrations were expressed in terms of the nominal concentrations of the active ingredient.
The study met the acceptability criteria prescribed by the protocol and was considered valid.
MEA Polyborate 1:3 reduced growth rate and inhibited the yield of this fresh water algae species significantly at 32 mg a.i./l and higher.
The EC50for growth rate reduction (ERC50: 0-72h) was 67 mg a.i./l with a 95 % confidence interval ranging from 52 to 85 mg a.i./l. This value is considered a short-term or acute endpoint under the OECD Guideline.
The EC50for yield inhibition (EYC50: 0-72h) was 32 mg a.i./l with a 95 % confidence interval ranging from 21 to 47 mg a.i./l.
The NOEC for both growth rate reduction and yield inhibition was 18 mg a.i./l. These values are considered long-term or chronic endpoints under the OECD Guideline.
Reference
Description of key information
Under the conditions of a GLP OECD 201 study with Pseudokirchneriella subcapitata, MEA Polyborate 1:3 reduced growth rate and inhibited the yield of this fresh water algae species significantly at 32 mg a.i./l and higher.
The EC50 for growth rate reduction (ERC50: 0-72h) was 67 mg a.i./l with a 95 % confidence interval ranging from 52 to 85 mg a.i./l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 67 mg/L
Additional information
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