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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

Based on a reliable study with diammonium hydrogenorthophosphat, the EC50 in Daphnia magna is determined to be >100 mg/L. 

Key value for chemical safety assessment

Additional information

In a reliable study performed according to OECD 202 (in 2016), 20 daphnids per concentration were exposed for 48 hours in a static set-up at 21°C to the following range of nominal test concentrations: 10, 18, 32, 56 and 100 mg/L. Test concentrations were analytically verified (ICP-MS) and found to a) be in the range 87 -92% of nominal and b) remain stable (based on P content) throughout the test preiod (98 -103% relative to initial). Therefore it is justified to base the endpoint on nominal test concentrations. Water quality parameters were maintained throughout the test.

 

The acute toxicity of DAP to Daphnia magna, expressed as EC50, was exceeding the highest tested concentration, thus 48 -h EC50 is >100 mg/L. This value shall be used for chemical safety assessment.

This result (not acutely toxic to D. magna) is in line with data available for an analogue substance from the phosphate category. In a reliable study performed according to "Standard Methods for the Examination of Water and wastewater (APHA-1975)", Daphnia carinata collected from local ponds were exposed to single superphosphate for 72 hr. Although the recommended exposure time is 48 hr, the extended exposure period is not expected to have affected the study as the LC50 values are high. LC50 values after 72 -hours of exposure at both 20.7°C and 30.8°C are 1790 and 1825 mg/L, respectively.