Diammonium hydrogenorthophosphate

Additional ecotoxological information

Administrative data

Endpoint:

additional ecotoxicological information

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

No data

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Limited information provided. The report is limited to the data mentioned in the summary. Since the fish were collected from a river, homogeneity of the population can not be guaranteed.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Evaluation of hematotoxic effects of two commonly used fertilizers, diammonium phosphate and urea, on fish Clarias batrachus.

Author:

Trivedi, S.P., Singh, P., Sethi, N. and Singh, R.K.

Year:

1990

Bibliographic source:

Ecotoxicology and environmental safety. 19: 135-142.

Reference 2

Reference Type:

publication

Title:

Impact of fertilizer, diammonium phosphate, on haematological profile of teleost fish, Heteropneutes fossilis.

Author:

Trivedi, S.P., Sethi, N., Singh, S.K. and Singh, P.

Year:

1989

Bibliographic source:

Bio. Mem. 15:166-174.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: No data

Deviations:

not specified

GLP compliance:

no

Type of study / information:

Type of test: Static system
Exposure period: 144/144/120/96/72/48/24 h for 0/0.81/0.86/0.92/0.97/1.01 and 1.10 g/L.
Analytical monitoring: No
TEST ORGANISMS
- Species: Clarias batrachus (Teleostei)
- Supplier: no
- Wild caught: river Gomati of Lucknow, India.

DILUTION WATER
- Source: tap water
- Alkalinity: 260-290 mg/L (Methyl orange)
- Hardness: 163-184 mg/L
- pH: 7.1-7.2
- O2: ≥80%

TEST SYSTEM
- Test type: Static
- Concentrations: 0, 0.81, 0.86, 0.92, 0.97, 1.01 and 1.10 g/L.
- Dosing rate: 0/day
- Renewal of test solution: no
- Exposure vessel type: glass aquarium
- Number of fish: 10 per vessel, 1 vessel/treatment
- Test duration: 144/144/120/96/72/48/24 h for 0/0.81/0.86/0.92/0.97/1.01 and 1.10 g/L.
- Test parameter: haemoglobin, RBC count, haematocrit and TLC.
- Observation times: at 24, 48, 72, 96, 120 and 144 h

PHYSICAL MEASUREMENTS
- Measuring times: 24, 48, 72, 96, 120 and 144 h
- Test temperature: 22.2-25.2°C
- Dissolved oxygen:>80%
- pH: 7.1-7.6
- Adjustment of pH: no

Test material

Test material information

Constituent 1

Reference substance name:

CAS 7783-28-0

IUPAC Name:

CAS 7783-28-0

Constituent 2

Reference substance name:

Diammonium hydrogenorthophosphate

EC Number:

231-987-8

EC Name:

Diammonium hydrogenorthophosphate

Cas Number:

7783-28-0

Molecular formula:

H3N.1/2H3O4P

IUPAC Name:

diammonium hydrogen phosphate

Details on test material:

Diammonium Phosphate, CAS 7783-28-0, nitrogen 18%, phosphoric acid 46%.
purity: not mentioned

Results and discussion

Any other information on results incl. tables

Results:

- Nominal concentrations (g/L): 0, 0.81, 0.86, 0.92,  0.97, 1.01 and 1.10 mg/L

- Mortality: not reported.

- Dose related effects: Hb, Hct, RBC and TLC.

- Other effects:

Conc. (g/L)	Ex-po-sure (h)		Mean Hb (g%)				Mean RBC (x10^6/cm^2)			Mean Hct (%)		Mean TLC (x10^3cells/cm^2)
			24h		end exposure		24h		end exposure	24h	end exposure	24h	end exposure
0	144	8.50[1]		n.m.[2]		3.56^1		n.m.^2		22.4	n.m.^2	22.9	n.m.^2
0.81	144	6.57		0.82		1.05		0.62		2.42	2.80	46.6	50.5
0.86	120	5.87		1.08		0.87		0.78		2.72	2.55	37.7	55.5
0.92	96	4.20		0.97		1.14		0.69		2.40	2.45	40.5	64.6
0.97	72	2.57		1.04		0.92		0.67		2.65	2.47	45.8	63.9
1.01	48	1.32		0.97		0.91		0.67		2.55	2.62	60.6	68.0
1.10	24	0.95		0.95		0.80		0.80		3.20	3.20	74.0	74.0

[1]Only measured at 72 h.

[2]Not measured.

Applicant's summary and conclusion

Conclusions:

At 0.81-1.1 g/L diammonium phosphate decreased Hb to 10-13%, Hct to 11-14% and RBC to 17-22% and increased TLC to 220-325% of 72h-control value.