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EC number: 404-450-2 | CAS number: 118685-34-0 COBRATEC 435
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 - 18 December 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- EEC Directive 67/548 Annex V C.1 as published ín 84/449/EEC
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Sodium 5-N-butylbenzotriazole
- EC Number:
- 404-450-2
- EC Name:
- Sodium 5-N-butylbenzotriazole
- Cas Number:
- 118685-34-0
- Molecular formula:
- CCCCc1ccc2c(c1)nnn2[Na]
- IUPAC Name:
- sodium 5-butyl-1H-1,2,3-benzotriazol-1-ide
- Test material form:
- solid - liquid: aqueous solution
- Details on test material:
- Identification: Butyl benzotriazole, sodium salt (Cobratec 435 - 37% aqueous solution)
Physical state: brown liquid
Analytical purity: 37%
Storage in original container at room temperature in darkness
Method of preparation: direct dispertion in water
Stability of test concentration: verified by chemical analysis. water sample taken from control, 0.7, 1.2, 3.7, 12 and 37 mg a.i./l at 0 and 48 hours and sent to the HRC Department of Analytical Chemistry for analysis by HPLC
Constituent 1
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In original container at room temperature in darkness
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: Sub-samples of the test solutions, following dilution with distilled water where necessary were analysed by high performance liquid chromatography.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Westacre Trout Farm, Norfolk
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 6.8 cm (SD = 0.4 cm)
- Weight at study initiation (mean and range, SD): 4.36 g (SD = 0.64 g)
- Method of breeding:
- Maintenance of the brood fish:
ACCLIMATION
- Acclimation period: 30 October 1989 - 27 November 1989
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food during acclimation: Commercial trout pellets
- Feeding frequency during acclimation: daily. Discontinued 24 h prior to test
- Health during acclimation (any mortality observed): Mortalities: <1% in 7 days prior to the test.
- Medication: None
- Temperature: 14 °C
- Dissolved oxygen: >= 10.1 mgO2/l
FEEDING DURING TEST
- Food type:
- Amount:
- Frequency:
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- Total hardness = 350 mg/l as CaCO3. This value is slightly higher than the recommended range but is not considered to have had any significant effects on the results of the test.
- Test temperature:
- 13°C
- pH:
- Concentration mg a.i./l 0 hours 24 hours 48 hours 72 hours 96 hours
pH pH pH pH pH
Control 7.1 7.1 7.1 7.2 7.2
0.67 7.1 7.2 7.2 7.2 7.1
1.2 7.1 7.2 7.2 7.2 7.2
2.1 7.1 7.2 7.2 7.2 7.2
3.7 7.1 7.3 7.3 7.2 7.2
6.7 7.1 7.4 - - - - Dissolved oxygen:
- Concentration mg a.i./l 0 hours 24 hours 48 hours 72 hours 96 hours
mgO2/l mgO2/l mgO2/l mgO2/l mgO2/l
Control 9.9 10.1 10.0 10.1 10.0
0.67 9.9 9.8 9.9 10.0 10.0
1.2 9.9 9.8 9.9 10.0 10.0
2.1 9.9 9.8 9.9 10.0 10.0
3.7 9.9 9.8 9.8 10.0 10.0
6.7 9.9 9.8 - - - - Salinity:
- see Appendix 3 test report
- Conductivity:
- see Appendix 3 test report
- Nominal and measured concentrations:
- 37% solution: 1.8, 3.2, 5.6, 10 and 18 mg/l
Active ingredient: 0.67, 1.2, 2.1, 3.7 and 6.7 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass Aquaria holding 40 l of test media
- Aeration: via narrow bore glass tubes
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water, dechlorinated by the addition of sodium thiosulphate. Total hardness = 350 mg/l as CaCO2. Thisvalue is slightly higher than the recommended range but is not considered to have had any significant effects on the results of the test.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8h dark
- Light intensity:
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight:
- Other biological observations: Marked reactions to exposure (other than death) were loss of equiligrium, swimming at surface, coughing and moribundity.
- Mortality of control: No
- Other adverse effects control:No
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Concentration mg a.i./l Abnormality 3 Hours 6 Hours 24 Hours 48 Hours 72 Hours 96 Hours Control No abnormalities detected 0.67 No abnormalities detected 1.2 No abnormalities detected 2.1 Swimming at surface 2/10 3/10 2/10 3.7 Coughing
Loss of equilibrium
Moribund
5/10
8/10
5/10
8/10
5/10
10/10
5/10
10/10
5/5
All fish dead 6.7 Moribund 1/1 All fish dead
Applicant's summary and conclusion
- Conclusions:
- Highest test concentration resulting in 0% mortality = 2.1 mg/l. Lowest test concentration resulting in 100% mortality = 3.7 mg/l.
The "no-observed effect concentration" is considered to be 1.2 mg/l. - Executive summary:
The acute toxicity (96 h LC50) of Butyl benzotriazole, sodium salt was tested on Rainbow trout in semi-static test conditions according to OECD Guideline No. 203 and EEC directive 67/548 Annex V C. l as published in 84/449/EEC.
Time (h) LC50 (mg/l) 95% confidence limits mg/l 3 5.1 4.4 - 6.0 6 5.0 3.7 - 6.7* 24 5.0 3.7 - 6.7* 48 5.0 3.7 - 6.7* 72 3.8 3.1 - 4.7 96 2.8 2.1 - 3.7* * Concentrations resulting in 0% mortality and 100 % mortality respectively.
Highest test concentration resulting in 0% mortality = 2.1 mg/l. Lowest test concentration resulting in 100% mortality = 3.7 mg/l.
The "no-observed effect concentration" is considered to be 1.2 mg/l.
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