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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1940
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1940

Materials and methods

Test guideline
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
Study performed before EU/OECD protocol establishment
GLP compliance:
no
Test type:
other:
Limit test:
no

Test material

Constituent 1
Reference substance name:
Ammonia, anhydrous
EC Number:
231-635-3
EC Name:
Ammonia, anhydrous
Cas Number:
7664-41-7
IUPAC Name:
ammonia
Details on test material:
Ammonia gas

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
Constant concentration of gas maintained by apparatus described by Setterstom and Zimmerman, 1938 and McCallan and Setterstom, 1940.
Mean temperature 73.3 ± 3.3 F, mean relative humidity 74.6 ± 8.8%.

Administration / exposure

Route of administration:
inhalation: gas
Type of inhalation exposure:
not specified
Vehicle:
not specified
Duration of exposure:
ca. 16 h
Concentrations:
1000ppm

Results and discussion

Preliminary study:
Rats exposed to 1000 ppm of NH3 were not noticeably affected till the end of the experiment of 16 h.
Rats continued to eat occasionally and climb around the cages during the experiments. Several began bedding at the end of the first hour and two older individuals showed some evidences of slight dyspnea. At the end of the exposure (16h) the eyes of all rats were bright with little or no evidence of lachrymation.

Two rats killed for autopsy showed all organs natural. Typical gross findings at autopsy of a rat which died 12 h after exposure showed findings in brain, lungs, heart, liver, stomach, intestine, adrenal and kidneys. Not remarkable findings were observed in trachea, gall bladder and peritoneal surfaces
Gas ammonia concentration tested was 1000 ppm.

The animals that survived the treatments and the controls were kept under observation for about 5 months.

Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
> 1 000 ppm
Based on:
test mat.
Exp. duration:
16 h
Mortality:
No mortalities were observed

Applicant's summary and conclusion

Executive summary:
Rats exposed to 1000 ppm of NH3 were not noticeably affected till the end of the experiment of 16 h. Rats continued to eat occasionally and climb around the cages during the experiments. Several began bedding at the end of the first hour and two older individuals showed some evidences of slight dyspnea. At the end of the exposure (16h) the eyes of all rats were bright with little or no evidence of lachrymation.   Two rats killed for autopsy showed all organs natural. Typical gross findings at autopsy of a rat which died 12 h after exposure showed findings in brain, lungs, heart, liver, stomach, intestine, adrenal and kidneys. Not remarkable findings were observed in trachea, gall bladder and peritoneal surfaces. The animals that survived the treatments and the controls were kept under observation for about 5 months

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