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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: basic information given; short study description; results only in tabular form

Data source

Reference
Reference Type:
publication
Title:
Acute Toxicity Tests on 113 Environmental Chemicals.
Author:
Hasegawa, B., Nakaji, Y., Kurokawa, Y., Tobe, M.
Year:
1989
Bibliographic source:
Sci. Rep. Res. Inst. Toboku Univ. 36, 10-16

Materials and methods

Principles of method if other than guideline:
The fifty percent of lethal dose (LD50) was calculated using the Litchfield and Wilcoxon method.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
highest purity available

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
Male and female animals (5 weeks of age) were used after an acclimatization for 1 week. Ten animals were used for each dosing and at
least 6 increasing dose levels were selected and tested to confirm the LD50 value.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
Test solutions were administered orally after 15 hours of food deprivation. Clinical signs and symptoms were monitored during the experimental period. Animals were given regular laboratory chow 6 hr after a single administration and the number of dead animals were counted until the 14th day. Anatomical examination was performed immediately after death or at terminal sacrifice on day 14.
The fifty percent of lethal dose was calculated using the Litchfield and Wilcoxon method.
Doses:
6 increasing dose levels
No. of animals per sex per dose:
total: 60
Control animals:
not specified

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
3 450 mg/kg bw
95% CL:
2 900 - 4 110
Sex:
female
Dose descriptor:
LD50
Effect level:
3 470 mg/kg bw
95% CL:
2 630 - 4 580
Mortality:
no data
Clinical signs:
hematuria, blood stool
Body weight:
no data
Gross pathology:
no data
Other findings:
congestion of stomach and brain

Applicant's summary and conclusion