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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Amines, tallow alkyl
EC Number:
263-125-1
EC Name:
Amines, tallow alkyl
Cas Number:
61790-33-8
IUPAC Name:
61790-33-8
Constituent 2
Reference substance name:
Amines, tallow alkyl
IUPAC Name:
Amines, tallow alkyl
Details on test material:
- Name of test material (as cited in study report): Genamin TA 100 D

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
The test substance was weighed out on quartz sand.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: own breeding, original source was the Bundesanstalt für Land und Forstwirtschaft.
- Weight at test initiation: 0.30 - 0.60 g

Housing:
- Housing conditions: Organisms were bred at the testing facility in covered plasitc vessels (volume 60 L) containing potting compost at 20 ± 2°C in the dark

ACCLIMATION
- Acclimation period: 1 day

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d

Test conditions

Test temperature:
20 ± 2 °C
Details on test conditions:
TEST SYSTEM
- Test container (material, size): glass dishes, 1 L volume
- Amount of soil or substrate:
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate):
10% peat
20% kaolin
70 % quartz sand
0.4 % calcium carbonate
- Organic carbon (%):
- Pretreatment of soil: deionised water was added to give a overall moisture content of about 25 % of dry weight. The definitive moisture content, determinated the day before application by drying a sample at 105 °C and re-weighing, was 16.3 %.



OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 400/800 lux

EFFECT PARAMETERS MEASURED : mortality and weight
Nominal and measured concentrations:
Nominal concatrations: control, 100, 180, 320, 580, 1000 mg/kg dry weight.
Reference substance (positive control):
yes
Remarks:
2-chloracetamide

Results and discussion

Effect concentrationsopen allclose all
Duration:
8 wk
Dose descriptor:
LC10
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
8 wk
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: LC 50 (14d) = 42.1 mg/kg

Any other information on results incl. tables

No mortality or singificant pathological symptoms were seen at the concentrations 100 -1000 mg/kg.

Applicant's summary and conclusion