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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012-01-12 to 2012-04-27
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Department of Health of the Government of the United Kingdom
Analytical monitoring:
yes
Details on sampling:
- Concentrations:all, at 0 and 48 hr
- Sample storage conditions before analysis: -20 degrees C until analysed
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dilution in test medium; serial dilution for concentrations.
- Controls: Test medium without test substance added
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Dapnia magna
- Source: in-house culture
- Age at study initiation (mean and range, SD): <24 hr
- Method of breeding: parthenogenesis
- Feeding during test: no

ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same as test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
None
Hardness:
250 mg/L CaCO3
Test temperature:
21-22 degrees C
pH:
7.7-8.0 SU
Dissolved oxygen:
Saturated
Nominal and measured concentrations:
Nominal- Control and 100 mg/L test substance
Measured concentrations were 96-102 % of nominal.
Details on test conditions:
TEST SYSTEM
- Test vessel: jar
- Type (delete if not applicable): closed; covered
- Material, size, headspace, fill volume: 250 mL glass, 200mL test volume
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted lab water used to make Elendt M7 standard Medium
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16:8 light:dark, with 20 minute dawn and dusk transitions
- Light intensity:662-723 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility, every 24 h

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.10, 1.0 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 1.1 mg/L, 95% CL 1.0-1.3 mg/L
- Other: results within normal ranges
Reported statistics and error estimates:
None- EC50 estimated by visual inspection
Validity criteria fulfilled:
yes
Conclusions:
The EC50 of the test substance to D. magna was found to be > 100 mg/L test substance.
Executive summary:

The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.

The study is classified as acceptable and satisfies guideline requirements for a acute toxicity study with freshwater invertebrates.

Results synopsis:

Test organism age: <24h

Test type: static

EC50 > 100 mg/L

NOEC: >= 100 mg/L

LOEC: > 100 mg/L

Endpoint(s) assessed: immobilisation

Description of key information

The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

Short-term toxicity of the test substance to invertebrates was investigated by exposing Daphnia magna (waterflea) during 48 hours in a static test, according to the OECD guideline 202. This resulted in an unbounded EC50 value of > 100 mg/L.