4-methylmorpholine 4-oxide, monohydrate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-01-12 to 2012-04-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Department of Health of the Government of the United Kingdom

Analytical monitoring:

yes

Details on sampling:

- Concentrations:all, at 0 and 48 hr
- Sample storage conditions before analysis: -20 degrees C until analysed

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct dilution in test medium; serial dilution for concentrations.
- Controls: Test medium without test substance added
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Dapnia magna
- Source: in-house culture
- Age at study initiation (mean and range, SD): <24 hr
- Method of breeding: parthenogenesis
- Feeding during test: no

ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): same as test

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

250 mg/L CaCO3

Test temperature:

21-22 degrees C

pH:

7.7-8.0 SU

Dissolved oxygen:

Saturated

Nominal and measured concentrations:

Nominal- Control and 100 mg/L test substance
Measured concentrations were 96-102 % of nominal.

Details on test conditions:

TEST SYSTEM
- Test vessel: jar
- Type (delete if not applicable): closed; covered
- Material, size, headspace, fill volume: 250 mL glass, 200mL test volume
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted lab water used to make Elendt M7 standard Medium
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16:8 light:dark, with 20 minute dawn and dusk transitions
- Light intensity:662-723 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility, every 24 h

TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.10, 1.0 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Mortality: yes
- EC50/LC50: 1.1 mg/L, 95% CL 1.0-1.3 mg/L
- Other: results within normal ranges

Reported statistics and error estimates:

None- EC50 estimated by visual inspection

Validity criteria fulfilled:

yes

Conclusions:

The EC50 of the test substance to D. magna was found to be > 100 mg/L test substance.

Executive summary:

The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.

The study is classified as acceptable and satisfies guideline requirements for a acute toxicity study with freshwater invertebrates.

Results synopsis:

Test organism age: <24h

Test type: static

EC₅₀ > 100 mg/L

NOEC: >= 100 mg/L

LOEC: > 100 mg/L

Endpoint(s) assessed: immobilisation

Description of key information

The 48-h toxicity of N-Methylmorpholine oxide to Daphnia magna was studied in an OECD 202 guideline study under static conditions. The study was conducted as limit test with a control ( 0 mg/L) and at 100 mg/L test substance (nominal concentration). No effects regarding immobilisation were observed. Therefore, the 48 h NOEC was >= 100 mg/L and the 48 h EC50 was > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short-term toxicity of the test substance to invertebrates was investigated by exposing Daphnia magna (waterflea) during 48 hours in a static test, according to the OECD guideline 202. This resulted in an unbounded EC50 value of > 100 mg/L.