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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

EL50 (invertebrates) = > 100 mg/L ; WAF loading 48hour-freshwater, OECD TG 202, WIL Research B.V. 2015

Key value for chemical safety assessment

Additional information

The acute toxicity to Daphnia magna was carried out according to OECD TG 202 Daphnia sp., Acute Immobilisation Test and EU Method C.2 guidelines. Twenty Daphnia magna per test group (4 replicates, 5 per vessel) were exposed to an untreated control group and to a WAF prepared at a test item loading rate of 100 mg/L. In the combined range-finding test ten daphnia per test group (2 replicates, 5 per vessel) were exposed to WAFs prepared at loading rates of 1.0 and 10 mg/L. All test concentrations were individually prepared and magnetically stirred for two days to ensure that maximum dissolution was obtained. This resulted in clear solutions all containing a floating layer of undissolved test item droplets. The Water Accommodated Fractions (WAFs) were subsequently collected after a three-hour stabilisation period. The final test solutions were all clear and colourless. A limit test was combined with a range-finding test. No significant effects on mobility of the organisms were observed at any of the WAFs tested. Samples for Total Organic Carbon (TOC) analyses were taken at the start and the end of the test period. TOC-analysis showed a measured organic carbon concentration of 0.75 mg C/L at the start of the test in the WAF prepared at a loading rate of 100 mg/L. The concentration measured after 48 hours had slightly decreased to 0.52 mg C/L. Analyses of the control group at the start and after 48 hours showed negligible concentrations of 0.09 and 0.06 mg C/L, respectively. These results should be considered as indicative only. The detection limit of the equipment was approximately 1 mg C/L and thus results at or below 1 mg C/L should be considered as estimates. The study met the acceptability criteria. The 48h-EL50 exceeded the WAF prepared at a loading rate of 100 mg/L and is therefore considered to be >100 mg/L.