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Environmental fate & pathways

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A study was conducted to assess the ready biodegradability of the test substance according to EU Method C.4-D (which is in most parts equivalent to OECD Guideline 301 F), in compliance with GLP. Activated sludge was inoculated with 100 mg/L of the test substance (in triplicate) in a closed flask at a constant temperature (22±2°C) for up to 28 d. Degradation was assessed by the determination of Biochemical Oxygen Demand (BOD) on Days 2, 6, 8, 10, 14, 16, 20, 22, 24 and 28. A control group, a reference substance group (100 mg/L benzoic acid, sodium salt) and a toxicity test (100 mg/L) were used for validation purposes. The test substance degraded 4% by Day 28. No toxicity of the test substance was observed. The reference compound showed 89% degradation at Day 14. Hence, under the study conditions, the test substance was considered to be ‘not readily biodegradable’ (Bruns, 2005).