3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study, non-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

EC Number:

226-006-5

EC Name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Cas Number:

5208-93-5

Molecular formula:

C15H24O

IUPAC Name:

(4E)-3-methyl-5-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Details on test material:

- Name of test material (as cited in study report): Vinyljonol
- Lot/batch No.: : not indicated
- Analytical purity: 94 %;
water solubility: poorly water soluble.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A concentrated stock solution of the test substance was prepared using Cremophor as a solvent. Then, a serial dilution of this stock solution in culture medium was prepared to obtain the desired test concentrations. In addition, a culture medium control and a solvent control was tested in parallel. The maximum solvent concentration in the test was 100 mg/l.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

18 - 22 °C, constant within +/-1 °C

pH:

8.0 - 8.2

Dissolved oxygen:

8.3 - 9.1 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at 400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 24 and 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.92 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

3.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Probit analysis.

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) / 0 / 24 / 48
Control /   0 /   0 /   0 
Solvent /   0 /   0 /   0 
0.4     /   0 /   5 /  15 
0.8     /   0 /   5 /  45 
1.6     /   0 /  15 / 100 
3.125   /   0 / 100 / 100 
6.25    /   0 / 100 / 100 
12.5    /   0 / 100 / 100 
25.0    /   0 / 100 / 100 
50.0    /   0 / 100 / 100 

24 hour ECx values:
EC0: 0.4 mg/l
EC50: 1.92 mg/l
EC100: 3.1 mg/l