3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study, GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

EC Number:

226-006-5

EC Name:

3-methyl-1-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Cas Number:

5208-93-5

Molecular formula:

C15H24O

IUPAC Name:

(4E)-3-methyl-5-(2,6,6-trimethylcyclohex-1-en-1-yl)penta-1,4-dien-3-ol

Details on test material:

- Name of test material (as cited in study report): Vinyljonol

Test animals / tissue source

Species:

rabbit

Strain:

Vienna White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: male: 3.28 kg; mean females: 3.40 kg
- Housing: single
- Diet: about 130 g per animal per day (Kliba 341)
- Water: about 250 ml tap water per animal per day
-Acclimation period: at least 8 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye of the same animal

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.1 ml

Duration of treatment / exposure:

see observation period (no washing out performed)

Observation period (in vivo):

72 hrs

Number of animals or in vitro replicates:

3 (one male, two females)

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: not performed

SCORING SYSTEM: according to OECD guideline 405/Draize

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Remarks:

animal 1-3

Time point:

other: 24/48/72 hrs

Score:

0

Max. score:

4

Reversibility:

other: no effect

Results 2

Irritation parameter:

iris score

Basis:

mean

Remarks:

animal 1-3

Time point:

other: 24/48/72 hrs

Score:

0

Max. score:

2

Reversibility:

other: no effect

Results 3

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 24/48/72 hrs

Score:

0.3

Max. score:

3

Reversibility:

fully reversible within: 48 hrs

Results 4

Irritation parameter:

conjunctivae score

Basis:

animal #2

Time point:

other: 24/48/72 hrs

Score:

0.3

Max. score:

3

Reversibility:

fully reversible within: 48 hrs

Results 5

Irritation parameter:

conjunctivae score

Basis:

animal #3

Time point:

other: 24/48/72 hrs

Score:

0.3

Max. score:

3

Reversibility:

fully reversible within: 48 hrs

Results 6

Irritation parameter:

chemosis score

Basis:

mean

Remarks:

animal 1-3

Time point:

other: 24/48/72 hrs

Score:

0

Max. score:

4

Reversibility:

other: no effect

Other effects:

no other effects observed

Any other information on results incl. tables

Animal	Reading	Corneal Opacity	Iris	Conjunctiva Redness	Chemosis
1	1 hr	0	0	2	0
2	1 hr	0	0	2	0
3	1 hr	0	0	2	0
1	24 hrs	0	0	1	0
2	24 hrs	0	0	1	0
3	24 hrs	0	0	1	0
1	48 hrs	0	0	0	0
2	48 hrs	0	0	0	0
3	48 hrs	0	0	0	0
1	72 hrs	0	0	0	0
2	72 hrs	0	0	0	0
3	72 hrs	0	0	0	0
mean animal 1-3	24/48/72 hrs	0.0	0.0	0.3	0.0