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EC number: 203-105-1 | CAS number: 103-37-7
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- P. M. JENNER et. al.
- Year:
- 1 964
- Bibliographic source:
- Food and Cosmetics Toxicology,1964
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- Richard J. Lewis
- Year:
- 1 989
- Bibliographic source:
- Food Additives Handbook , pp 83,1989
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- D. Belsito et. al.
- Year:
- 2 012
- Bibliographic source:
- Food and Chemical Toxicology ,2012
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- U.S. National Library of Medicine
- Year:
- 2 018
- Bibliographic source:
- Chemidplus Database,U.S. National Library of Medicine,2018
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- T.B.Adams et. al.
- Year:
- 2 005
- Bibliographic source:
- Food and Chemical Toxicology,2005
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- D. L. J. Opdyke
- Year:
- 1 974
- Bibliographic source:
- Food and Cosmetics Toxicology, 1974
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- Richard J. Lewis
- Year:
- 2 012
- Bibliographic source:
- Sax's Dangerous Properties of Industrial Materials, 12th Edition, 5 Volume Set,2012
- Reference Type:
- other: secondary source
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- RTECS
- Year:
- 2 018
- Bibliographic source:
- RTECS (registry of toxic effect of chemical substance database), 2018
- Reference Type:
- publication
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- D. McGinty et. al.
- Year:
- 2 012
- Bibliographic source:
- Food and Chemical Toxicology ,2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Acute Oral toxicity test was carried out to study the effects of test chemical on rats.
- GLP compliance:
- not specified
- Test type:
- other: No data available
Test material
- Reference substance name:
- Benzyl butyrate
- EC Number:
- 203-105-1
- EC Name:
- Benzyl butyrate
- Cas Number:
- 103-37-7
- Molecular formula:
- C11H14O2
- IUPAC Name:
- benzyl butanoate
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report): Benzyl butyrate
- IUPAC NAME: Butanoic acid, phenylmethyl ester
- Molecular formula : C11H14O2
- Molecular weight : 178.23 g/mole
- Smiles notation : c1(COC(CCC)=O)ccccc1
- InChl : 1S/C11H14O2/c1-2-6-11(12)13-9-10-7-4-3-5-8-10/h3-5,7-8H,2,6,9H2,1H3
- Substance type: Organic
- Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Osborne-Mendel
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: young and adult rats
- Fasting period before study: 18 hours approx
- Diet (e.g. ad libitum): the food was replaced in cages as soon as animals received their respective doses.
- Water (e.g. ad libitum): ad libitum
Administration / exposure
- Route of administration:
- other: intubation
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- The test material was applied undiluted
- Doses:
- 2330 mg/kg
- No. of animals per sex per dose:
- 10:animals
5 : males
5 : females - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: No
- Other examinations performed: clinical signs, body weight, mortality - Statistics:
- LD50's were computed by the method of Litchfield & Wilcoxon (1949)
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 2 330 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 1 940 - <= 2 800
- Remarks on result:
- other: 50% mortality was observed
- Mortality:
- Mortality were observed in 4hrs-4 days
- Clinical signs:
- other: Depression, scrawny appearance, tremors with higher doses
- Gross pathology:
- Necropsy was not conducted
- Other findings:
- no data available
Any other information on results incl. tables
Acute oral toxicity of food flavourings and compounds of related structure
CAS |
Species |
LD50 with 95 % confidence limits (mg/kg) |
Slope function with 95% confidence limits |
Toxic sign and death time (D.T) |
103 -37 -7 |
Rat |
2330 (1940-2800) |
1:3 (1.1- 1.6) |
Depression, scrawny appearance, tremors with higher doses D.T. 4hrs-4 days |
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified
- Conclusions:
- The lethal concentration (LD50) value for acute oral toxicity test was considered to be 2330 mg/kg bw (95% confidence limit:1940-2800),when group of 5 male and female Osborne-Mendel rats were treated with test chemical orally.
- Executive summary:
Acute oral toxicity study was performed in groups of 10 young adult Osborne-Mendel rats evenly divided by sexusing test chemical at dose concentration of 2330 mg/kg bw.All doses were given by intubation. All animals were maintained under close observation for recording toxic signs and time of death. Such observation was continued until animals appeared normal and showed weight gain. The usual observation period was 2 weeks. LD50's were computed by the method of Litchfield & Wilcoxon (1949).The substance was a liquid and it was administered undiluted.50% mortality was observed at dose 2330 mg/kg bw.Clinical signs like somnolence (general depressed activity), Scrawny appearance, tremors at higher doses were observed. Hence,LD50 value was considered to be 2330 mg/kg bw (1940-2800) with 95% confidence limits,when rats were treated withtest chemical orally.
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