Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
bioaccumulation in aquatic species: invertebrate
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Influence of glyphosate and its formulation (Roundup) on the toxicity and bioavailability of metals to Ceriodaphnia dubia
Author:
Martin T.K. Tsui, Wen-Xiong Wang , L.M. Chu ,
Year:
2005
Bibliographic source:
Environmental Pollution 138 (2005) 59-68

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Data is from peer reviewed journal
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Mercury sulphate
EC Number:
231-992-5
EC Name:
Mercury sulphate
Cas Number:
7783-35-9
Molecular formula:
H2O4S.Hg
IUPAC Name:
mercury sulfate
Constituent 2
Reference substance name:
Sulfuric acid, Mercury(2+) salt(1:1)
IUPAC Name:
Sulfuric acid, Mercury(2+) salt(1:1)
Details on test material:
- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data available
Radiolabelling:
yes

Sampling and analysis

Details on sampling:
No data available

Test solutions

Vehicle:
not specified
Details on preparation of test solutions, spiked fish food or sediment:
Details on preparation of test solutions or sediment (aquatic)
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: No data available
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No data available
- Concentration of vehicle in test medium (stock solution and final test solution(s) at different concentrations and in control(s)): No data available
- Evidence of undissolved material (e.g. precipitate, surface film, etc): No data available

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
Details on test organisms
TEST ORGANISM
- Common name: Water flea
- Strain: No data available
- Source: Aquatic Research Organisms (Hampton, NH, USA)
- Age at study initiation (mean and range, SD): Adults Ceriodaphnia dubia
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: No data available
- Food type: Selenastrum capricornutum and yeastecerophyle
trout chow
- Amount: No data available
- Frequency: 2

ACCLIMATION
- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food:
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Study design

Route of exposure:
aqueous
Test type:
not specified
Water / sediment media type:
not specified
Total exposure / uptake duration:
4 h

Test conditions

Hardness:
No data available
Test temperature:
No data available
pH:
No data available
Dissolved oxygen:
No data available
TOC:
No data available
Salinity:
No data available
Details on test conditions:
Details on test conditions
TEST SYSTEM
- Test vessel: polypropylene beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100ml
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: 10 to 20
individuals
- No. of vessels per concentration (replicates): No data available
- No. of vessels per control / vehicle control (replicates): No data available
- Biomass loading rate: No data available

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductance: No data available
- Holding medium different from test medium: No data available
- Intervals of water quality measurement: No data available
- Intervals of test medium replacement: No data available

OTHER TEST CONDITIONS
- Adjustment of pH: 25°C
- Photoperiod: No data available
- Light intensity: No data available

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: No data available
- Results used to determine the conditions for the definitive study: No data available
Nominal and measured concentrations:
Nominal: 5000, 10000, 15000, 20000 L kg-1)
Reference substance (positive control):
not specified
Details on estimation of bioconcentration:
No data available

Results and discussion

Bioaccumulation factor
Type:
other: No data available
Value:
> 0 - < 5 000
Basis:
other: Bioconcentration
Remarks on result:
other: Conc.in environment / dose:No data available
Details on kinetic parameters:
No data available
Metabolites:
No data available
Results with reference substance (positive control):
No data available
Details on results:
No data available
Reported statistics:
No data available

Applicant's summary and conclusion

Conclusions:
The bioconcentration value for the Ceriodaphnia dubia was found to be (> 0 to < 5000) value when treated with Sulfuric acid, Mercury (2+) salt (1:1) (7783-35-9).
Executive summary:

The bioconcentration test was performed on Ceriodaphnia dubia when treated with Mercury (2+) salt (1:1). Adults were used and two treatments were given as glyphosate was mixed with203Hg (0.71 nM) in the

diluent water for 30 min in a polypropylene beaker. Another only metal solution was stir for 30 minutes. After 4 hours of exposure Ceriodaphnia dubia were collected and check for radioactivity.

From the above experiment it was found that the bioconcentration value for the Ceriodaphnia dubia was found to be (> 0 to < 5000) value when treated with Sulfuric acid, Mercury (2+) salt (1:1) (7783-35-9).