2,4-diaminobenzenesulphonic acid

Short-term toxicity to fish

Description of key information

The predicted data  from QSAR model suggests the lethal concentration (LC50) for the 2,4-Diaminobenzenesulfonic acid was estimated to be 274.723480225 mg/L on fish Pimephales promelas on the basis of mortality effect.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

274.723 mg/L

Additional information

Predicted model data and experimental study result for the test chemical 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1) with respective read across were reviewed to summarize the following information:

In key study the 96 hrs aquatic fish toxicity study was predicted to assess toxic effects of the test compound 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1)and the result were predicted (SSS QSAR Prediction model, 2016). The study was based on the effects of the test compound on Pimephales promelas fish in a static fresh water system. The predicted data suggests the lethal concentration (LC50) for the test compound was estimated to be 274.723480225 mg/l on the basis of mortality effect.

Whereas supporting model which is based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours LC50 was estimated to be 14900000 mg/l on fish for test substance 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1)with mortality effects.

Based on above predicted value from SSS QSAR and ECOSAR, it can be concluded that the substance can be considered as non toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Above predicted lethal concentrations supported by experimental study (Chemosphere. Vol. 28, No. 12, pp. 2203.2236, 1994)for read across chemical Sulfanilic acid (Cas no. 121-57-3) which is indicates that Toxic effects of test substance 4-amino-benzenesulfonic acid was determined on various fish species like Leuciscus idus, Bruchydunio rerio,Salmo gaidneri, Pimephales promelas, Lepomis macrochirus, Poecilia reticulate for 48 and 96 hrs in static condition and in fresh water.

During experiment the lethal concentrations i.e LC0 and LC50 values were found to be 1000 mg/l and 100.4 mg/l respectively.

Different experimental data (J. Hydrology; 51:359-367; 1981) for same read across i.e Sulfanilic acid (Cas no. 121-57-3) was indicate toxic effects of test chemical Sulfanilic acid on fish (Pimephales promelas) in static fresh water with study condition of 40-48 mg/L CaCO3 hardness,22±1°C test temp.,7.2-7.9 pH,> 40 % Sat dissolved oxygen and 120 to 160 uS/cm conductivity.

During study the lethal concentration (LC50) was found to be 100.4 mg/l on the basis of mortality effect.

Another read across 3-methylsulphanilic acid (Cas no. 98-33-9) from experimental study indicate that the acute toxicity to fish test been performed to evaluate the toxicological effects of chemical 4-Amino-m-toluenesulfonic acid in fish. The chemical was exposed to Gambusia affinis fish with dose concentration of 180, 320, 560, 1000 mg/L with exposure duration of 24, 48 and 96 hrs.The mortality was observed in the fish, at 560 and 1000 mg/L all animals were dead in 6 hrs only.Hence, the LC50 value was found to be 375 mg/L, 425 mg/L and 410mg/L when with duration of 96, 24 and 48-hrs respectively when tested in Gambusia affinis fish based on mortality. (Sewage and Industrial Wastes Vol. 29, No. 6 (Jun., 1957), pp. 695-711)

Whereas authorative database (Data bank of Environmental Properties of Chemicals (EnviChem), 2016) for read across 4-aminotoluene-3-sulphonic acid (Cas no. 88-44-8) indicate that toxic effects was examined on fish Oryzias latipes for 48 hrs.

During experiment the lethal concentration (LC50) was found to be 480 mg/l on the basis of mortality effects.

Thus, based on above all lethal concentration values, it can be concluded that the target chemical 2,4-Diaminobenzenesulfonic acid (CAS No. 88-63-1) can be considered as non-toxic to fish and thus cannot be classified as hazardous as per the CLP criteria.