1H-Pyrazole-3-carbonitrile, 4,4'-dithiobis[5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-09-19 - 1995-09-26

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Remarks:

spacing factor was 3.0

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile

EC Number:

603-434-4

Cas Number:

130755-46-3

Molecular formula:

C22 H8 Cl4 F6 N8 S2

IUPAC Name:

5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile

Details on test material:

- Name of test material (as cited in study report): MB 046307
- Physical state: yellow powder
- Analytical purity: 972 g/kg
- Lot No.: 95-4023
- Storage condition of test material: The test substance was stored in the dark in an air-tight container at room temperature (approximately 20°C).

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent): Dimethylformamide (DMF)
- Concentration of vehicle in test medium (final test solutions including solvent control): 0.1 ml/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Presence of undissolved particles only in higher concentrations (0.22, 0.67, 2.0 mg/L)

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Batch no.: 95T081
- Source: Salmonids hatchery, Charles River Aquatics, 76410 St-Aubin-les-Elbeuf, France
- Age at study initiation: < 1 year
- Length at study initiation (mean, range and SD): 4.4 cm (4.0 - 5.0 cm)
- Weight at study initiation (mean and range, SD): 0.55 g (0.36 - 0.85 g)
- Feeding during test: no

ACCLIMATION
- Acclimation period: a minimum of 12 days
- Acclimation conditions: same as test
- Type and amount of food: commercial trout food pellets
- Feeding frequency: twice daily except during the last 24 hours prior to testing
- Health during acclimation (any mortality observed): no mortality the last 7 days before test initation
- Photoperiod: artificial light, 16 hours light and 8 hours darkness.

Study design

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

40 ± 10 mg/L (CaCo3)

Test temperature:

14.0 - 14.5 °C

pH:

7.1 - 7.4

Dissolved oxygen:

8.9 - 9.8 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: control, 0.02, 0.07, 0.22, 0.67, 2.0 mg/L
- Mean measured test concentrations: < 0.01, 0.008, 0.03, 0.21, 0.52, 2.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, fill volume: 20 L glass aquaria, 15 L
- Aeration: No aeration during test period.
- Type of flow-through: peristaltic
- Renewal rate of test solution: 50 ml/min
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.08 g/L per 24 hours


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water filtered through active charcoal and diluted with reverse osmosed deionized water
- Conductivity: 110 ± 20 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 h, 48 h, 96 h


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light and 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, physical or behavioral alterations after 24, 48, 72, 96 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Range finding study
- A preliminary test was performed over a period of 72 hours for which the LC50 was estimated to be in excess of the highest tested concentration of 3.0 mg/l. Based on the preliminary test the nominal concentrations were calculated according to a geometric series by a factor of 3

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: above the visual limit of aqueous solubility

Details on results:

- Behavioural abnormalities: accelerated respiration, lethargy, muscular contractions, partial paralysis
- Other biological observations: pigmentation disorders
- Mortality of control: none
- Other adverse effects control (solvent control): lethargy, partial paralysis