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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
January 25, 2016 ~ April 06, 20 16
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-[cyclohexane-1,1-diylbis(4,1-phenyleneoxymethylene)]dioxirane
Cas Number:
13446-84-9
Molecular formula:
C24H28O4
IUPAC Name:
2,2'-[cyclohexane-1,1-diylbis(4,1-phenyleneoxymethylene)]dioxirane
Test material form:
liquid: viscous

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The toxicity of the test item was determined by preparing a water-accommodated fractions (WAF) of the test item. The water-accommodated fraction was applied to dilution water containing only the fraction of multi-component test item that was dissolved and/or present as
a stable dispersion. The preparation method for the WAF was as follows:
0.235 g of the test item (purity: 85%) was added to a 2000 mL beaker and dilution water was added up to 2000 mL mark of the beaker to give the nominal loading rate of 100 mg/L. The prepared test solution was stirred for 48 hours using magnetic stirrer. Undissolved test item existed on the wall of the beaker because the test item was very sticky.
After 48 hours of mixing, the middle part of solution was siphoned off after settling for 30 minutes to achieve the WAF from the nominal 100 mg/L test item loading rate. WAF of l 00 mg/L solution was regarded as 100 mg/L of nominal concentration and 67, 45, 30, 20 mg/L of test solutions were prepared by serial dilution method.
A I 00 mL portion of prepared each solution was distributed to tlu·ee replicate 250 mL volume Erlenmeyer flasks. The dilution water (algae culture medium) only was used for the control group.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static

Test conditions

Test temperature:
21.7
pH:
8.0
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (total fraction)
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 72ru-ECso values expressed in terms of the nominal loading rate of SEZ-250 to Pseudokirchneriella subcapitαta were > 100 mg/L for average growth rate and 53 .5 mg/L for yield.
The No Observed Effect Concentration (NOEC) and Lowest Observed Effect Concentration (LOEC) values expressed in terms of the nominal loading rate were 30 mg/Land 45 mg/L for average growth rate and yield, respectively.