p-(dimethoxymethyl)anisole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study. Scientifically acceptable.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412 L15

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2108-92-7

IUPAC Name:

2108-92-7

Details on test material:

- Name of test material (as cited in study report): Anisaldehyddimethylacetal, raw
- Lot/batch No.: J1 - L23
- Analytical purity: 85 - 90 %
water solubility: < 2000 mg/L according to sponsor.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers, 2354 Hohenweststadt, Germany
- Length at study initiation (length definition, mean, range and SD): 5.2 cm (range 4.3 - 6.0 cm)
- Weight at study initiation (mean and range, SD): 1.3 g (range 0.7 - 2.1 g)
- Corpulence factor of the test fish batch: 0.9
- Housing and adaption: Culture conditions: oil-free aerated and charcoal filtered tap water, flow-through system; Water temperature: 20 - 22 °C Duration of housing: about 1 month Mortality during the last 2 weeks of housing: 0.03 % , Medical treatment: Twice with 0.05 mg/L malachite green chloride, once with 10 mg/L tetracycline hydrochloride
- Feeding during test: 1 day before and during exposure

ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food:Growing feed F/B 50, ad libitum
- Health during acclimation (any mortality observed): no mortality observed

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 °C

pH:

7.3 - 7.8

Dissolved oxygen:

8.1 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 46.4, 100.0, 215.0, 464.0 and 1000.0 mg/L (based on the results of a range-finding test). In addition, a control was tested in parallel.

Details on test conditions:

TEST SYSTEM
- Test vessel: All glass aquaria (30 x 22 x 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 1.3 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Test water: reconstituted freshwater according to DIN 38412, Part II, 1982; prepared from fully demineralised tap water (conductivity: max 10 micro MHO) by adding: 294.0 mg/L CaCl2 * 2 H2O, 123.3 mg/L MgSO4 + 7 H2O, 63.0 mg/L NaHCO3, 5.5 mg/L KCl
- Ratio Na/K ions: 10 : 1
- Ca/mg ratio: 4:1
- Acid capacity: 0.8 mmol/L


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

316 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

466 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

48 h-LC50 of the positive control Chloroacetamide: about 31 mg/L (corresponds to normal sensitivity).

Reported statistics and error estimates:

The EC50 value was calculated as the geometrical mean of 215 mg/L (LC0) and 464 mg/L (LC100) value.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to Anisaldehyddimethyldiacetal, raw, mortalities were as follows:

Nominal test Cumulative mortalitiy (%) after x hours
concentration 1 / 4 / 24 / 48 / 72 / 96
(mg/L)
Control 0 / 0 / 0 / 0 / 0 / 0
46.4 0 / 0 / 0 / 0 / 0 / 0
100.0 0 / 0 / 0 / 0 / 0 / 0
215.0 0 / 0 / 0 / 0 / 0 / 1
464.0 0 / 4 / 10 / 10 / 10 / 10
1000.0 1 / 10 / 10 / 10 / 10 / 10

Symptoms of intoxication:

Nominal test Symptoms of intoxication after x hours
concentration 1 / 4 /24 / 48 / 72 / 96
(mg/L)
Control - / - / - / - / - / -
46.4 - / - / - / - / - / -
100.0 - / - / - / - / - / -
215.0 A / A / A / 0 / T / -
464.0 T / N / + / + / + / +
1000.0 N / + / + / + / + / +

Definitions of Symptoms:
-: No symptoms
+: All fish dead
A: Apathy
N: Narcotic-like state
T: Tumbling


Behavior of the test item during the test:
Undissolved oily test substance was visible on the bottom of the test aquaria.