p-(dimethoxymethyl)anisole

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline-study, non-GLP; purity of test item <= 90 %

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38 412 L11,

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

p-(dimethoxymethyl)anisole

EC Number:

218-577-4

EC Name:

p-(dimethoxymethyl)anisole

Cas Number:

2186-92-7

Molecular formula:

C10H14O3

IUPAC Name:

1-(dimethoxymethyl)-4-methoxybenzene

Details on test material:

- Name of test material (as cited in study report): Anisaldehyddimethylacetal, raw
- Lot/batch No.: I 1 - L 23
- Analytical purity: about 85 - 90 %; content: 85 - 90 % Anisaldehyddimethylacetal, 6 - 13 % medium to highly volatile compounds
water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A concentrated stock solution of nominal 500 mg/l was prepared using a 30 min stirring period at 21 °C.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 4 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19 - 21 °C

pH:

7.3 - 8.1

Dissolved oxygen:

4.5 - 9.1 mg/l

Nominal and measured concentrations:

Nominal test concentration (mg/l): 15.62, 31.2, 62.5, 125, 250, 580 and control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the later guideline DIN 38412, L 11


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours
- Light intensity: diffuse light (10 microeinstein/m*m*s) at 400 - 750 nm wave length


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

31.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

31.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

85.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

Probit analysis.

Any other information on results incl. tables

No of immobile daphnids after x hours in %:
Concentration (mg/L) 0 / 3 / 6 / 24 / 48
Control / 0 / 0 / 5 / 5 / 5
15.62 / 0 / 0 / 0 / 5 / 10
31.2 / 0 / 0 / 0 / 5 / 10
62.5 / 0 / 0 / 0 / 15 / 25
125 / 0 / 5 / 5 / 10 / 75
250 / 0 / 0 / 0 / 25 / 100
580 / 0 / 25 / 40 / 70 / 100

24 hour ECx values:
EC0: 31.2 mg/l
EC50: 375.2 mg/l
EC100: > 500 mg/l