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Diss Factsheets

Administrative data

Key value for chemical safety assessment

Additional information

1. SUMMARY FROM HSDB for CAS 119 -53 -9 (benzoin)

Benzoin was found to be negative when tested for mutagenicity using the

Salmonella/microsome preincubation assay, using the standard protocol

approved by the National Toxicology Program (NTP). Benzoin was tested in

as many as 6 Salmonella typhimurium strains (TA1535, TA1537, TA1538, TA97, TA98,

and TA100) in the presence and absence of rat and hamster liver S-9, at

doses of 0.033, 0.0333, 0.100, 0.333, 0.3333, 0.667, 1.000, and 3.3333

mg/plate. The highest ineffective dose tested in any S. typhimurium strain

was 3.3333 mg/plate. Precipitate was observed in most cultures at the high

dose and also some clearing of the background bacterial lawn was also

seen. [Mortelmans K et al; Environ Mutagen 8:1-119 (1986)]

... Levels of Evidence of Carcinogenicity: Male Rats: Negative; Female

Rats: Negative; Male Mice: Negative; Female Mice: Negative. [Bioassay of

Benzoin for Possible Carcinogenicity (1980) Technical Rpt Series No. 204

DHEW Pub No. (NIH) 80-1760, U.S. Department of Health Education and

Welfare, National Cancer Institute, Bethesda, MD 20014]

A bioassay of benzoin for possible carcinogenicity was conducted by

incorporating the test chemical in diets of F344 rats and B6C3F1 mice. ...

Groups of 50 male rats were fed diets containing 125 or 250 ppm benzoin

for 104 wk, and similar groups of female rats received feed containing 250

or 500 ppm. Groups of 50 mice if each sex were fed diets containing 2,500

or 5,000 ppm benzoin for 104 wk. Groups of 50 untreated rats and mice of

each sex were used as matched controls. ... Under the conditions of this

bioassay, benzoin was not carcinogenic in F344 rats or B6C3F1 mice. Levels

of Evidence of Carcinogenicity: Male Rats: Negative; Female Rats:

Negative; Male Mice: Negative; Female Mice: Negative. [Bioassay of Benzoin

for Possible Carcinogenicity (1980) Technical Rpt Series No. 204 DHEW Pub

No. (NIH) 80-1760, U.S. Department of Health Education and Welfare,

National Cancer Institute, Bethesda, MD 20014]

2. OECD Toolbox experimental data

Benzoin was found positive in an in vitro mammalian chromosome aberration test (Chinese hamster lung cells)

without metabolic activation, negative in the in vivo mouse micronucleus test and negative in a mammalian

in vitro cell gene mutation assay (mouse lymphoma cells).


Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

At the found effect levels there is no requirement for classification