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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
2004
Deviations:
no
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzyltris(dimethylaminato)phosphorus(1+) tetrafluoroborate(1-)
EC Number:
302-080-5
EC Name:
Benzyltris(dimethylaminato)phosphorus(1+) tetrafluoroborate(1-)
Cas Number:
94088-77-4
Molecular formula:
C13H25BF4N3P
IUPAC Name:
benzyltris(dimethylaminato)phosphorus(1+) tetrafluoroborate(1-)
Test material form:
solid: crystalline

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Chemical analysis of the test item concentration in the test solutions was performed to determine exposure concentration and stability of the test item during exposure. Substance specific analysis was performed at the test site. The samples were filled into 2 mL microcentrifuge tubes and stored in the freezer (≤ -18 °C). The
sampling was conducted according to the following specification:
• At the start of the test (0 h), the test solutions NC, A, C and E were sampled before division to the test vessels (each group: 4 samples of 1 mL).
• After 24 h exposure, all replicates of NC, A, C and E were sampled (each group: 4 samples of 1 mL). Also from group B and D, 1 mL test solution per replicate are removed and discarded.
• At the end of the test (48 h), all replicates from NC, A, C and E were sampled (each group: 4 samples of 1 mL).
Of each sampled treatment, one of the samples from 0 h, 24 h and 48 h was handed over to the analytical laboratory at the test site and analysed. The remaining samples were stored as retain samples in the freezer until finalization of the study. Detailed information of the analytical method is given in the analytical phase report.

Test solutions

Vehicle:
no
Details on test solutions:
The stock solution was prepared by adding 32.0 mg test item to 1000 mL test medium and stirring for 30 min using a magnetic stirrer at room temperature.
This stock solution was used as highest test item concentration in the test.
Into each test vessel, 20 mL test item solution and test medium (negative control; NC) was transferred, respectively. Four replicates with five daphnids each,
were prepared for each test treatment and NC. The further test item concentrations were prepared by diluting the stock solution with test medium.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: clone breeding of the Federal Environment Agency, department IV 2.4, Berlin, and has been cultivated at Hydrotox GmbH since October 2012.
- Age of parental stock (mean and range, SD): The test organisms used were 0.25 - 23.50 h old at the start of the test.
- Feeding during test: fed on workdays with Desmodesmus subspicatus algae corresponding to 0.1 mg C × daphnid-1 × day-1.

ACCLIMATION

The daphnids are cultured at 20 ± 2 °C with 16 h light : 8 h dark and placed into fresh M4 medium twice a week.
They are kept at approx. 10 animals / 200 mL.

Quality assurance takes place at regular intervals by testing the sensitivity of the test organisms to the reference item potassium dichromate
(Sigma, Steinheim, Germany; Lot no.: MKBF2111V, expiration date: January 2017).
The recent quality testing was performed in November 2016 with EC50 (24h) = 1.36 mg/L (CL 95 %: 1.28 - 1.44 mg/L) and EC50 (48h) < 0.84 mg/L,
which is in the declared range of 0.6 - 2.1 mg/L (24 h) according to OECD 202.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19.3 - 20.8°C
pH:
7.2 - 7.9 in the control and 7.4 - 7.9 in the test item treatment
Dissolved oxygen:
7.8 - 8.3 mg/L in the control and 7.8 - 8.1 mg/L in the test item treatment
Nominal and measured concentrations:
2, 4, 8, 16, 32 mg/l.
The measured test item concentrations in the test item treatments were 100.25 – 107.50 % of the nominal test item concentration
and stable during the exposure period. The test item concentrations in the control were below the limit of quantification.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
21.82 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
14.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
other: EC20
Effect conc.:
16.99 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
>= 32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test is valid according to OECD Test Guideline 202 (April 2004). The measured test item concentrations in the test item treatments were 100.25 – 107.50 % of the nominal test item concentration and stable during the exposure period. The test item concentrations in the control were below the limit of quantification. Immobilisation of the test organisms in the control at the end of the test was 0 % and therefore ≤ 10 %. Dissolved oxygen concentration in the control and the test item treatment at the end of the test was ≥ 7.8 mg/L and therefore ≥ 3 mg/L.

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