Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-507-3 | CAS number: -
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Remarks:
- alternative method
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Annex VI of French Official Journal n°302 of 30/12/1999
- Deviations:
- not specified
- Principles of method if other than guideline:
- Determination of the cytotoxicity after contact of the test element (5 dilutions) with a monolayer of cells marked by a vital dye (Neutral Red) by calculation of the percentage of cell death and the IC50 or concentration of test element that inhibited of 50 % the survival and cell growth
- GLP compliance:
- no
Test material
- Reference substance name:
- Polysaccharids of kernel Avena sativa, Poaceae
- EC Number:
- 944-507-3
- IUPAC Name:
- Polysaccharids of kernel Avena sativa, Poaceae
- Reference substance name:
- Water
- EC Number:
- 231-791-2
- EC Name:
- Water
- Cas Number:
- 7732-18-5
- Molecular formula:
- H2O
- IUPAC Name:
- oxidane
- Test material form:
- liquid
- Details on test material:
- Osilift is the commercilized product
Appearance: light clear yellow liquid
Storage conditions: 20°C in a dark place
Constituent 1
additive 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: SIRC (ATCC -CCL60)
- Remarks:
- Cornea fibroblasts
Results and discussion
In vitro
Results
- Irritation parameter:
- in vitro irritation score
- Remarks:
- cellular mortality CI50
- Run / experiment:
- One on one
- Value:
- > 50 - <= 100
- Vehicle controls validity:
- not specified
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- no indication of irritation
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The irritant potential of the product Osilift towards rabbit cornea fibroblasts was determined at the concentrations of 5 to 50%. The IC50 of Osilift is higher than 50%. The per cent mortality observed at the dilution of 50% is to 48%. The cytotoxicity of Osilift is thus slight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
