Tricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers

EC Number:

249-236-8

EC Name:

Dichlorotricyclo[8.2.2.24,7]hexadeca-1(12),4,6,10,13,15-hexaene, mixed isomers

Cas Number:

28804-46-8

Molecular formula:

C16H14Cl2

IUPAC Name:

Unknown constituents

Test material form:

solid: granular

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

acetone

Details on test solutions:

Before conducting the preliminary range finding study, test item was checked for solubility and precipitation. Test item was found to be insoluble in reconstituted water at the concentration of 0.1 mg/mL. Further solubility was checked in dimethyl formamide (DMF), acetone and Dimethyl sulfoxide (DMSO) and found to be soluble at 33.33 (Stock A), 28.57 (Stock B) and 22.22 (Stock C) mg/mL, respectively. Maximum solubility of test item found in dimethyl formamide (DMF) and acetone. Hence, test item soluble in DMF and acetone was further serially diluted in respective solvents and checked for precipitation in the test medium.
Stock solution A was serially diluted at the concentration of 16.66 (Stock D), 8.33 (Stock E), 4.16 (Stock F), and 2.08 (Stock G) mg/mL in DMF. These stock solutions were checked for precipitation test using reconstituted water. Precipitation was observed upto the concentration of 4.16 mg/mL, while no precipitation was observed at the concentrations of 2.08 mg/mL.
Stock solution B was serially diluted at the concentration of 14.29 (Stock H), 7.14 (Stock I) and 3.57 (Stock J) mg/mL in acetone. These stock solutions were checked for precipitation test using reconstituted water. Precipitation was observed upto the concentration of 7.14 mg/mL, while no precipitation was observed at the concentrations of 3.57 mg/mL.
Hence, the Acetone was used as vehicle for the study. And the highest concentration was used 0.357 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.357 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

As the study was conducted as a limit test of 0.357 mg DPX-C (Di-Cloro-Di-p-Xililene)/L and no immobility observed up to 48 h, 24 and 48 h, EC50 could not be calculated and was considered to be greater than 0.357 mg DPX-C (Di-Cloro-Di-p-Xililene)/L.

Executive summary:

This study was performed to assess the toxic effects ofDPX-C (Di-Cloro-Di-p-Xililene) exposureonDaphnia magnaunder static conditionsaccording to the OECD guideline 202.

Based on the results of the preliminary range finding study, the main study was restricted to limit study and it was performed at the dose level of 0.357 mg/L along with control and vehicle control groups. In the main study, a 48 h static toxicity test procedure was followed, comprising six groups. Each group consisted of four replicates with 5 daphnids per replicate.

The stability of the test item in the test media was also performed during method validation (JRF Study N° 228-2-13-14896). The test media was analysed for DPX-C (Di-Cloro-Di-p-Xililene) active ingredient concentration to monitor the concentration of test solution at 0 and 48 during main study. The active ingredient concentration of the test item in the test media was within the acceptable limit (80% of nominal concentration).

As the study was conducted as a limit test of 0.357 mgDPX-C (Di-Cloro-Di-p-Xililene)/L and no immobility observed up to 48 h, 24 and 48 h, EC₅₀could not be calculated and was considered to be greater than 0.357 mgDPX-C (Di-Cloro-Di-p-Xililene)/L.